Detailed information of TA system TA169730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 110234..110910
• Replicon: plasmid p1
• Accession: NZ_CP060037
• Organism: Sphingobium barthaii strain KK22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7M2GQU3
• Locus tag: H5V43_RS21900
• Protein ID: WP_025548987.1
• Coordinates: 110234..110656 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7M2GPS4
• Locus tag: H5V43_RS21905
• Protein ID: WP_025548988.1
• Coordinates: 110656..110910 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5V43_RS21865	105367..105591	-	225	WP_025548977.1	hypothetical protein	-
H5V43_RS21870	105950..106636	-	687	WP_025548979.1	hypothetical protein	-
H5V43_RS21875	106838..107824	-	987	WP_025548980.1	DUF2493 domain-containing protein	-
H5V43_RS21880	108122..108367	-	246	WP_025548982.1	hypothetical protein	-
H5V43_RS21885	108486..109004	-	519	WP_025548983.1	DUF736 domain-containing protein	-
H5V43_RS21890	109259..109573	-	315	WP_025548984.1	hypothetical protein	-
H5V43_RS21895	109615..110166	-	552	WP_052816688.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
H5V43_RS21900	110234..110656	-	423	WP_025548987.1	type II toxin-antitoxin system VapC family toxin	Toxin
H5V43_RS21905	110656..110910	-	255	WP_025548988.1	Arc family DNA-binding protein	Antitoxin
H5V43_RS21910	110996..111655	-	660	WP_025548989.1	hypothetical protein	-
H5V43_RS21915	111677..113473	-	1797	WP_150293340.1	PcfJ domain-containing protein	-
H5V43_RS21920	113470..114081	-	612	WP_150293338.1	hypothetical protein	-
H5V43_RS21925	114261..114476	-	216	WP_025548995.1	hypothetical protein	-
H5V43_RS21930	114580..115098	-	519	WP_025548996.1	hypothetical protein	-
H5V43_RS21935	115178..115537	-	360	WP_025548998.1	DUF3768 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..387592	387592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-45)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15277.59 Da
• Isoelectric Point: 7.0118

>T169730 WP_025548987.1 NZ_CP060037:c110656-110234 [Sphingobium barthaii]
MIVVDTNVISEMAKPQPSISVRRWLDAQPFDALYLTTITMAEMRFGIMVLPASKRRSFLEAALERTEQLYAGRILSFDQD
AARHYADLATLARAKGKGFPAPDAYIAAIAAANGFIVSTRDTAPFEAAGLGVINPWLSTN

Nucleotide

• Length: 423 bp

>T169730 NZ_CP076687:537185-537487 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9183.39 Da
• Isoelectric Point: 5.7084

>AT169730 WP_025548988.1 NZ_CP060037:c110910-110656 [Sphingobium barthaii]
MASVTVRNIPDEVHRALRVRAAQHGRSTEAEIRIILEQAAMPEGRRLMGSALSEIWGTAGLTEEEAAAIEGARDRRPATP
MSFD

Nucleotide

• Length: 255 bp

>AT169730 NZ_CP076687:537523-537864 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7M2GQU3

Antitoxin

• AlphaFold DB: A0A7M2GPS4