Detailed information of TA system TA169424

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 155195..155621
• Replicon: plasmid p1
• Accession: NZ_CP059918
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: H5C78_RS25650
• Protein ID: WP_001302184.1
• Coordinates: 155195..155353 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	155397..155621 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS25620	150369..150764	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
H5C78_RS25625	150863..151552	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
H5C78_RS25630	151739..152122	-	384	WP_001151524.1	relaxosome protein TraM	-
H5C78_RS25635	152442..153044	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
H5C78_RS25640	153341..154162	-	822	WP_001234487.1	DUF945 domain-containing protein	-
H5C78_RS25645	154273..154569	-	297	WP_001272245.1	hypothetical protein	-
H5C78_RS25650	155195..155353	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	155397..155621	-	225	NuclAT_0	-	Antitoxin
-	155397..155621	-	225	NuclAT_0	-	Antitoxin
-	155397..155621	-	225	NuclAT_0	-	Antitoxin
-	155397..155621	-	225	NuclAT_0	-	Antitoxin
H5C78_RS25655	155633..156352	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
H5C78_RS25660	156349..156783	-	435	WP_072834353.1	conjugation system SOS inhibitor PsiB	-
H5C78_RS25665	156838..158796	-	1959	WP_171995101.1	ParB/RepB/Spo0J family partition protein	-
H5C78_RS25670	158860..159093	-	234	WP_000005988.1	DUF905 family protein	-
H5C78_RS25675	159056..159688	-	633	WP_039023645.1	single-stranded DNA-binding protein	-
H5C78_RS25680	159989..160414	+	426	WP_015387398.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..214538	214538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T169424 WP_001302184.1 NZ_CP059918:c155353-155195 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T169424 NZ_CP076471:202588-202854 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGACAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAGAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT169424 NZ_CP059918:c155621-155397 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin