Detailed information of TA system TA169369

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102034..102288
• Replicon: plasmid p1
• Accession: NZ_CP059908
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H5C80_RS25650
• Protein ID: WP_001351576.1
• Coordinates: 102034..102240 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	102227..102288 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS25615	97576..97923	+	348	Protein_95	IS1-like element IS1A family transposase	-
H5C80_RS25620	98363..98650	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5C80_RS25625	98647..98898	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
H5C80_RS25630	99862..100719	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H5C80_RS25635	100712..101194	-	483	WP_001273588.1	hypothetical protein	-
H5C80_RS25640	101187..101261	-	75	WP_001442103.1	RepA leader peptide Tap	-
H5C80_RS25645	101493..101750	-	258	WP_000083845.1	replication regulatory protein RepA	-
H5C80_RS25650	102034..102240	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	102227..102288	+	62	NuclAT_1	-	Antitoxin
-	102227..102288	+	62	NuclAT_1	-	Antitoxin
-	102227..102288	+	62	NuclAT_1	-	Antitoxin
-	102227..102288	+	62	NuclAT_1	-	Antitoxin
H5C80_RS25655	102544..102618	-	75	Protein_103	endonuclease	-
H5C80_RS25660	102864..103076	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H5C80_RS25665	103212..103772	-	561	WP_000139315.1	fertility inhibition protein FinO	-
H5C80_RS25670	103875..104735	-	861	WP_000704513.1	alpha/beta hydrolase	-
H5C80_RS25675	104794..105540	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucB / iucA	1..193291	193291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T169369 WP_001351576.1 NZ_CP059908:c102240-102034 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T169369 NZ_CP076448:1285204-1285593 [Elioraea tepida]
GTGATCGTCGACACCTCCGCCCTGCTCGCCATCCTTCTGGGCGAGGAGGAGGCCGCGCGCTTCGCCTCCGCCATCCTCGA
CGCCCCGAGCGCCGGAATCTCGGTCGCGAACGTCCTCGAGACGGCGATCGTTCTGGAGCGGCGCGGCAACGCGCTGGCGC
GCAGCCGGCTCGATCCGCTGGTTGCCGATCTCGGCCTCGCCTTCCTGCCCGTCACCGTCGCCCAGATGCACCGCGCCCGC
GCCGCCTTCACTCGGTTCGGCAAGGGCCGCCATCCCGCCGGCCTCAACTTCGGCGACTGCTTCGCCTATGCGCTCGCCGA
GGAGACGGGCGAGCCGCTCCTGTTCAAGGGCGAGGACTTCGCGCGGACGGACATCGCCCGCGCGCTCTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT169369 NZ_CP059908:102227-102288 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin