Detailed information of TA system TA169367

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5102647..5103445
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X1M0U2
• Locus tag: H5C80_RS25085
• Protein ID: WP_000854919.1
• Coordinates: 5102647..5103024 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2G4AEZ5
• Locus tag: H5C80_RS25090
• Protein ID: WP_001285608.1
• Coordinates: 5103071..5103445 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS25060	5098147..5098326	+	180	WP_001153034.1	hypothetical protein	-
H5C80_RS25065	5098725..5100761	-	2037	WP_000417002.1	hypothetical protein	-
H5C80_RS25070	5101714..5101860	-	147	WP_001290177.1	hypothetical protein	-
H5C80_RS25075	5101945..5102142	-	198	WP_001403937.1	DUF957 domain-containing protein	-
H5C80_RS25080	5102162..5102650	-	489	WP_000777665.1	hypothetical protein	-
H5C80_RS25085	5102647..5103024	-	378	WP_000854919.1	TA system toxin CbtA family protein	Toxin
H5C80_RS25090	5103071..5103445	-	375	WP_001285608.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H5C80_RS25095	5103525..5103746	-	222	WP_000692350.1	DUF987 domain-containing protein	-
H5C80_RS25100	5103833..5104309	-	477	WP_001186188.1	RadC family protein	-
H5C80_RS25105	5104324..5104803	-	480	WP_000706980.1	antirestriction protein	-
H5C80_RS25110	5104903..5105043	+	141	WP_000997937.1	hypothetical protein	-
H5C80_RS25115	5105069..5105887	-	819	WP_001175165.1	DUF945 domain-containing protein	-
H5C80_RS25120	5105977..5106210	-	234	WP_001278293.1	DUF905 family protein	-
H5C80_RS25125	5106216..5106893	-	678	WP_001097301.1	hypothetical protein	-
H5C80_RS25130	5107041..5107721	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	5091490..5104803	13313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14037.97 Da
• Isoelectric Point: 7.9086

>T169367 WP_000854919.1 NZ_CP059907:c5103024-5102647 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T169367 NZ_CP076445:516925-517275 [Bacillus subtilis subsp. subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13610.37 Da
• Isoelectric Point: 5.5051

>AT169367 WP_001285608.1 NZ_CP059907:c5103445-5103071 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPAITS

Nucleotide

• Length: 375 bp

>AT169367 NZ_CP076445:516639-516920 [Bacillus subtilis subsp. subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A1X1M0U2

Antitoxin

• AlphaFold DB: A0A2G4AEZ5