Detailed information of TA system TA169360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3323642..3325224
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0J2BJD7
• Locus tag: H5C80_RS16145
• Protein ID: WP_001125469.1
• Coordinates: 3323642..3324958 (-)
• Length: 439 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H5C80_RS16150
• Protein ID: WP_001296726.1
• Coordinates: 3324958..3325224 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS16130	3321031..3321741	-	711	WP_001195165.1	fimbria/pilus periplasmic chaperone	-
H5C80_RS16135	3322103..3322666	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
H5C80_RS16140	3323100..3323366	-	267	Protein_3144	helix-turn-helix transcriptional regulator	-
H5C80_RS16145	3323642..3324958	-	1317	WP_001125469.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
H5C80_RS16150	3324958..3325224	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H5C80_RS16155	3325417..3327306	-	1890	Protein_3147	autotransporter-associated beta strand	-
H5C80_RS16160	3327456..3329048	-	1593	WP_000113152.1	autoinducer-2 kinase	-
H5C80_RS16165	3329127..3330080	-	954	WP_000154356.1	transcriptional regulator LsrR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 439 a.a.
• Molecular weight: 49275.91 Da
• Isoelectric Point: 8.8783

>T169360 WP_001125469.1 NZ_CP059907:c3324958-3323642 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPQVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREWY

Nucleotide

• Length: 1317 bp

>T169360 NZ_CP076412:957444-957743 [Ralstonia wenshanensis]
ATGCTGACGATCCGCACCACCAACGCTTTCGACACCTGGTTTTCGAGGCTGCGTGACAAGACTGCGCAGCGCCGTATCCA
GGTGCGCATCGACCGCTTGCAACTGGGTCATCCAGGTGATGTGAAGGCTGTCCGAGCTGGTGTCAGTGAACTACGCATCA
ATCACGGCCCCGGCTACCCGGTGTATTTCGTCCAGCGTGGCGCAGTACTGATCATCCTGTTATGTGGCGGTGACAAGTCG
ACACAGGAAGCCGACATCCGACGCGCGATTGAGTTGTCGCGCCATCTCGACGTGGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT169360 WP_001296726.1 NZ_CP059907:c3325224-3324958 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT169360 NZ_CP076412:957746-958048 [Ralstonia wenshanensis]
ATGCCTAACGACAAACCCGTCAAAACCCGCCCGTGGGACTCCGCCGAGCATCTCAAGACCGAGGCCGATATGGCCGCATA
TCTGGACGCGTGCCTGGAGGAAGCGGCCGACGACCCCGCCTTTATCACCCATGCGCTGGGCGTGGTGGCCCGCGCGCGCG
GCATGACACAACTTGCGCGTGATACCGGCATGACGCGGGAAGGGCTGTACAAGGCCTTGTCCGAAGAAGGCAACCCGAGC
TTTGCCACTGTGCTCAAAGTCATGCGGGCGTTGGGTATTCGCTTGCATGCGGTCGCGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2BJD7