Detailed information of TA system TA169355

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3142859..3143230
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: H5C80_RS15275
• Protein ID: WP_001317028.1
• Coordinates: 3143036..3143230 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3142859..3143037 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS15250	3138814..3140187	+	1374	WP_001272254.1	ATP-dependent RNA helicase DbpA	-
H5C80_RS15255	3140316..3141251	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
H5C80_RS15260	3141303..3142538	-	1236	WP_000040852.1	site-specific integrase	-
H5C80_RS15265	3142540..3142755	-	216	WP_000079604.1	excisionase XisR	-
H5C80_RS15270	3142834..3143043	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	3142859..3143037	+	179	NuclAT_0	-	Antitoxin
-	3142859..3143037	+	179	NuclAT_0	-	Antitoxin
-	3142859..3143037	+	179	NuclAT_0	-	Antitoxin
-	3142859..3143037	+	179	NuclAT_0	-	Antitoxin
H5C80_RS15275	3143036..3143230	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
H5C80_RS15280	3143287..3144096	-	810	WP_000166319.1	recombination protein RecT	-
H5C80_RS15285	3144089..3146689	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
H5C80_RS15290	3146791..3147066	-	276	WP_000632297.1	protein RacC	-
H5C80_RS15295	3147141..3147311	-	171	WP_001352098.1	conserved protein, Rac prophage	-
H5C80_RS15300	3147311..3147532	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3141303..3189459	48156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T169355 WP_001317028.1 NZ_CP059907:c3143230-3143036 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T169355 NZ_CP076407:c4234705-4234391 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGAATACTAAATTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT169355 NZ_CP059907:3142859-3143037 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin