Detailed information of TA system TA169343

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1590727..1591525
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: H5C80_RS07810
• Protein ID: WP_042037557.1
• Coordinates: 1590727..1591104 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: H5C80_RS07815
• Protein ID: WP_001285415.1
• Coordinates: 1591151..1591525 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS07780	1586677..1587942	-	1266	WP_046644379.1	integrase arm-type DNA-binding domain-containing protein	-
H5C80_RS07785	1588406..1588732	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H5C80_RS07790	1588729..1588992	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
H5C80_RS07795	1589064..1589930	-	867	WP_042630955.1	DUF4942 domain-containing protein	-
H5C80_RS07800	1590027..1590224	-	198	WP_000839266.1	DUF957 domain-containing protein	-
H5C80_RS07805	1590242..1590730	-	489	WP_000761680.1	hypothetical protein	-
H5C80_RS07810	1590727..1591104	-	378	WP_042037557.1	TA system toxin CbtA family protein	Toxin
H5C80_RS07815	1591151..1591525	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H5C80_RS07820	1591605..1591826	-	222	WP_000692309.1	DUF987 domain-containing protein	-
H5C80_RS07825	1591889..1592365	-	477	WP_001366855.1	RadC family protein	-
H5C80_RS07830	1592381..1592860	-	480	WP_042630954.1	antirestriction protein	-
H5C80_RS07835	1592942..1593760	-	819	WP_001175149.1	DUF945 domain-containing protein	-
H5C80_RS07840	1593850..1594083	-	234	WP_001278287.1	DUF905 family protein	-
H5C80_RS07845	1594089..1594766	-	678	WP_001097301.1	hypothetical protein	-
H5C80_RS07850	1594914..1595594	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14033.01 Da
• Isoelectric Point: 7.8044

>T169343 WP_042037557.1 NZ_CP059907:c1591104-1590727 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T169343 NZ_CP076405:c4235939-4235625 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGAATACTAAATTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT169343 WP_001285415.1 NZ_CP059907:c1591525-1591151 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT169343 NZ_CP076405:c4235640-4235356 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Structures

Antitoxin

• AlphaFold DB: A0A067H947