Detailed information of TA system TA169342

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1563462..1564294
• Replicon: chromosome
• Accession: NZ_CP059907
• Organism: Escherichia coli strain 181.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: H5C80_RS07665
• Protein ID: WP_000854753.1
• Coordinates: 1563462..1563836 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H5C80_RS07670
• Protein ID: WP_182928215.1
• Coordinates: 1563926..1564294 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C80_RS07630	1558530..1559135	+	606	WP_001318033.1	type II secretion system minor pseudopilin GspJ	-
H5C80_RS07635	1559132..1560109	+	978	WP_000633196.1	type II secretion system minor pseudopilin GspK	-
H5C80_RS07640	1560106..1561284	+	1179	WP_000097200.1	type II secretion system protein GspL	-
H5C80_RS07645	1561286..1561822	+	537	WP_000942807.1	GspM family type II secretion system protein YghD	-
H5C80_RS07650	1562102..1562671	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
H5C80_RS07655	1562768..1562965	-	198	WP_000839252.1	DUF957 domain-containing protein	-
H5C80_RS07660	1562977..1563465	-	489	WP_001549830.1	hypothetical protein	-
H5C80_RS07665	1563462..1563836	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
H5C80_RS07670	1563926..1564294	-	369	WP_182928215.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H5C80_RS07675	1564374..1564595	-	222	Protein_1487	DUF987 domain-containing protein	-
H5C80_RS07680	1564658..1565134	-	477	WP_001186710.1	RadC family protein	-
H5C80_RS07685	1565146..1565625	-	480	WP_000860065.1	antirestriction protein	-
H5C80_RS07690	1565707..1566525	-	819	WP_001234631.1	DUF945 domain-containing protein	-
H5C80_RS07695	1566624..1566857	-	234	WP_001278287.1	DUF905 family protein	-
H5C80_RS07700	1566863..1567540	-	678	WP_001097301.1	hypothetical protein	-
H5C80_RS07705	1567688..1568368	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T169342 WP_000854753.1 NZ_CP059907:c1563836-1563462 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T169342 NZ_CP076405:c4235640-4235356 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13531.44 Da
• Isoelectric Point: 6.6290

>AT169342 WP_182928215.1 NZ_CP059907:c1564294-1563926 [Escherichia coli]
VSDTLPGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT169342 NZ_CP076405:c4235939-4235625 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATTAAAAACCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAATAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTTCTTTTTGGTGAAGTTGAGGGGGAACTATGAATACTAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0