Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1124054..1124691 Replicon chromosome
Accession NZ_CP059855
Organism Bacillus velezensis strain Pm9

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag H4K32_RS05935 Protein ID WP_003156187.1
Coordinates 1124054..1124404 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag H4K32_RS05940 Protein ID WP_003156188.1
Coordinates 1124410..1124691 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4K32_RS05895 1119094..1119696 - 603 WP_003156170.1 SpoIIE family protein phosphatase -
H4K32_RS05900 1119696..1120484 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
H4K32_RS05905 1120450..1120932 - 483 WP_003156173.1 anti-sigma B factor RsbW -
H4K32_RS05910 1120929..1121258 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
H4K32_RS05915 1121322..1122329 - 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
H4K32_RS05920 1122341..1122742 - 402 WP_003156178.1 anti-sigma regulatory factor -
H4K32_RS05925 1122745..1123110 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
H4K32_RS05930 1123115..1123936 - 822 WP_014304404.1 STAS domain-containing protein -
H4K32_RS05935 1124054..1124404 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
H4K32_RS05940 1124410..1124691 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
H4K32_RS05945 1124811..1125980 - 1170 WP_003156189.1 alanine racemase -
H4K32_RS05950 1126097..1127104 - 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
H4K32_RS05955 1127269..1127634 - 366 WP_014304402.1 holo-ACP synthase -
H4K32_RS05960 1127727..1128326 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T169285 WP_003156187.1 NZ_CP059855:c1124404-1124054 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T169285 NZ_CP076367:c18637-18329 [Gemmobacter fulva]
TTGAAAAGACTGGTCATTGCAGAGCCTGCAGCCCGCGATCTTGAGGGGATTGTAAACTATATCGCACTGGATAACCCCGC
CGCCGCAGAGACAGTATATCGGGGCATCGTAGAAACGGCGAGGAAACTGCCGCAGTTCCCAGCTCTCGGGCGACCTGGTC
GCCACCCGGAGACACGAGAAATGAGTGTTTCCGGTTTGCCTTACTTGATTGTTTATGAAGTTGGTGCCGAGACGGTCACG
ATCTTGGCGGTGTTTCACACGGCCCGCGATCTCGCCCAGGCGCTGCGCGAAAGGCTGATAGCCAGCTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT169285 WP_003156188.1 NZ_CP059855:c1124691-1124410 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT169285 NZ_CP076367:c18906-18634 [Gemmobacter fulva]
ATGATTACAAAGGATCACAAAATGCCTCAGCGGCCTACAACCGAGCCGATTACCATCCGCACGGCAAAGGTTCAGGAAAT
CGACGCCTTGGCACATGCGATGGACCGGTCACGCAACTACATCGTCAATCAGGCGATCGAGCAGTATCTGGAAGCCAACG
TCTGGCAGATGGAACGTATCTCTGCAGGGATCGCCGCAGCGCGTGAAGGCAAGGTTTTGCCTGCCGATGATGTCTTCGCC
GCTATTGCCGCGAAGCATGGCTGGTCGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References