Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 10952..11474 Replicon plasmid pCF3863_tetX
Accession NZ_CP059850
Organism Citrobacter freundii strain 3863

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag H4O05_RS23795 Protein ID WP_000220560.1
Coordinates 11193..11474 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag H4O05_RS23790 Protein ID WP_000121743.1
Coordinates 10952..11203 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4O05_RS23765 (H4O05_23680) 6623..6904 + 282 WP_001185482.1 hypothetical protein -
H4O05_RS23770 (H4O05_23685) 6929..7906 + 978 WP_024245156.1 hypothetical protein -
H4O05_RS23775 (H4O05_23690) 7903..8259 - 357 WP_000456533.1 DNA distortion polypeptide 3 -
H4O05_RS23780 (H4O05_23695) 8396..8842 - 447 Protein_10 hypothetical protein -
H4O05_RS23785 (H4O05_23700) 8882..9718 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
H4O05_RS23790 (H4O05_23705) 10952..11203 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
H4O05_RS23795 (H4O05_23710) 11193..11474 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H4O05_RS23800 (H4O05_23715) 11549..12246 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
H4O05_RS23805 (H4O05_23720) 12392..12649 + 258 WP_105278499.1 hypothetical protein -
H4O05_RS23810 (H4O05_23725) 12686..13654 - 969 WP_077783444.1 IS5 family transposase -
H4O05_RS23815 (H4O05_23730) 13674..13964 + 291 WP_073503549.1 peptidoglycan DD-metalloendopeptidase family protein -
H4O05_RS23820 (H4O05_23735) 13998..14093 - 96 Protein_18 DUF2933 domain-containing protein -
H4O05_RS23825 (H4O05_23740) 14158..14862 + 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(X4) - 1..51531 51531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T169268 WP_000220560.1 NZ_CP059850:11193-11474 [Citrobacter freundii]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T169268 NZ_CP076344:c1987163-1987056 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT169268 WP_000121743.1 NZ_CP059850:10952-11203 [Citrobacter freundii]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT169268 NZ_CP076344:1987216-1987277 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References