Detailed information of TA system TA169241

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2983111..2983906
• Replicon: chromosome
• Accession: NZ_CP059840
• Organism: Escherichia coli strain E2348/69

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: H4J01_RS15050
• Protein ID: WP_000854914.1
• Coordinates: 2983111..2983485 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7UP42
• Locus tag: H4J01_RS15055
• Protein ID: WP_001280955.1
• Coordinates: 2983532..2983906 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4J01_RS15010	2978116..2978607	-	492	WP_001340063.1	DUF1097 domain-containing protein	-
H4J01_RS15015	2978709..2979263	-	555	WP_001001921.1	molecular chaperone YcdY	-
H4J01_RS15020	2979287..2980024	-	738	WP_000283657.1	zinc-binding phosphatase	-
H4J01_RS15025	2980079..2981017	-	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
H4J01_RS15035	2981487..2982329	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
H4J01_RS15040	2982414..2982611	-	198	WP_000839282.1	DUF957 domain-containing protein	-
H4J01_RS15045	2982623..2983114	-	492	WP_000976857.1	hypothetical protein	-
H4J01_RS15050	2983111..2983485	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
H4J01_RS15055	2983532..2983906	-	375	WP_001280955.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H4J01_RS15060	2984069..2984290	-	222	WP_001220314.1	DUF987 domain-containing protein	-
H4J01_RS15065	2984353..2984829	-	477	WP_001186738.1	RadC family protein	-
H4J01_RS15070	2984845..2985330	-	486	WP_000214398.1	antirestriction protein	-
H4J01_RS15075	2985421..2986239	-	819	WP_001234664.1	DUF945 domain-containing protein	-
H4J01_RS15080	2986579..2987649	-	1071	WP_000102671.1	patatin-like phospholipase family protein	-
H4J01_RS15085	2987646..2988551	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T169241 WP_000854914.1 NZ_CP059840:c2983485-2983111 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T169241 NZ_CP076318:779535-779711 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13767.59 Da
• Isoelectric Point: 6.6248

>AT169241 WP_001280955.1 NZ_CP059840:c2983906-2983532 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT169241 NZ_CP076318:779757-780173 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9P0D0