Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 16813..17353 Replicon plasmid pPC997_1
Accession NZ_CP059577
Organism Legionella sp. PC997

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G4RL43
Locus tag HBNCFIEN_RS16500 Protein ID WP_014845117.1
Coordinates 17033..17353 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2H5FRI5
Locus tag HBNCFIEN_RS16495 Protein ID WP_014845118.1
Coordinates 16813..17046 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBNCFIEN_RS16470 11942..14440 + 2499 WP_014845123.1 DEAD/DEAH box helicase family protein -
HBNCFIEN_RS16475 14620..14901 - 282 WP_014845122.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
HBNCFIEN_RS16480 14894..15154 - 261 WP_014845121.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
HBNCFIEN_RS16485 15367..16251 + 885 WP_014845120.1 recombinase family protein -
HBNCFIEN_RS16490 16357..16704 + 348 WP_080031944.1 WGR domain-containing protein -
HBNCFIEN_RS16495 16813..17046 + 234 WP_014845118.1 hypothetical protein Antitoxin
HBNCFIEN_RS16500 17033..17353 + 321 WP_014845117.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HBNCFIEN_RS16505 17791..18183 - 393 Protein_19 methyltransferase domain-containing protein -
HBNCFIEN_RS16510 18549..19082 - 534 WP_061637559.1 IS91 family transposase -
HBNCFIEN_RS16515 19104..19595 - 492 WP_051009158.1 IS91 family transposase -
HBNCFIEN_RS16520 19667..19822 + 156 WP_154080667.1 hypothetical protein -
HBNCFIEN_RS16525 20187..20774 + 588 WP_014845114.1 Hsp20/alpha crystallin family protein -
HBNCFIEN_RS16530 20922..21413 + 492 WP_014845113.1 Hsp20/alpha crystallin family protein -
HBNCFIEN_RS16535 22039..22341 + 303 WP_014845112.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-29 - 1..150431 150431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11444.32 Da        Isoelectric Point: 9.4321

>T168998 WP_014845117.1 NZ_CP059577:17033-17353 [Legionella sp. PC997]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Download         Length: 321 bp

>T168998 NZ_CP076268:4244445-4244756 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8932.14 Da        Isoelectric Point: 4.8047

>AT168998 WP_014845118.1 NZ_CP059577:16813-17046 [Legionella sp. PC997]
MNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Download         Length: 234 bp

>AT168998 NZ_CP076268:4244757-4245047 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G4RL43

Antitoxin

Source ID Structure
AlphaFold DB A0A2H5FRI5

References