Detailed information of TA system TA168997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 14620..15154
• Replicon: plasmid pPC997_1
• Accession: NZ_CP059577
• Organism: Legionella sp. PC997

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7G4RL39
• Locus tag: HBNCFIEN_RS16475
• Protein ID: WP_014845122.1
• Coordinates: 14620..14901 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7G4RL40
• Locus tag: HBNCFIEN_RS16480
• Protein ID: WP_014845121.1
• Coordinates: 14894..15154 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBNCFIEN_RS16450	10039..10362	+	324	WP_014845127.1	hypothetical protein	-
HBNCFIEN_RS16455	10415..11233	+	819	WP_014845126.1	hypothetical protein	-
HBNCFIEN_RS16460	11272..11625	+	354	WP_014845125.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HBNCFIEN_RS16465	11603..11893	+	291	WP_014845124.1	helix-turn-helix domain-containing protein	-
HBNCFIEN_RS16470	11942..14440	+	2499	WP_014845123.1	DEAD/DEAH box helicase family protein	-
HBNCFIEN_RS16475	14620..14901	-	282	WP_014845122.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
HBNCFIEN_RS16480	14894..15154	-	261	WP_014845121.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HBNCFIEN_RS16485	15367..16251	+	885	WP_014845120.1	recombinase family protein	-
HBNCFIEN_RS16490	16357..16704	+	348	WP_080031944.1	WGR domain-containing protein	-
HBNCFIEN_RS16495	16813..17046	+	234	WP_014845118.1	hypothetical protein	-
HBNCFIEN_RS16500	17033..17353	+	321	WP_014845117.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HBNCFIEN_RS16505	17791..18183	-	393	Protein_19	methyltransferase domain-containing protein	-
HBNCFIEN_RS16510	18549..19082	-	534	WP_061637559.1	IS91 family transposase	-
HBNCFIEN_RS16515	19104..19595	-	492	WP_051009158.1	IS91 family transposase	-
HBNCFIEN_RS16520	19667..19822	+	156	WP_154080667.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..150431	150431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11028.69 Da
• Isoelectric Point: 8.9816

>T168997 WP_014845122.1 NZ_CP059577:c14901-14620 [Legionella sp. PC997]
MLELELATQFKRDLKKITKQGKRRTLLDSIVEQLQKEEPLDPKHKDHSLSVNWDGYRECHITPDWLLIYKTIKDDRIQLL
RLSRTGSHSDLFG

Nucleotide

• Length: 282 bp

>T168997 NZ_CP076268:c3773281-3773051 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9594.08 Da
• Isoelectric Point: 6.9803

>AT168997 WP_014845121.1 NZ_CP059577:c15154-14894 [Legionella sp. PC997]
MNKAATINARIEPALKMQAEAILHKVGLSTAEAIRLFYSQVCLQNGLPFEVKIPNKETREAMAELESGKGERFKTMKDVW
DSVDNA

Nucleotide

• Length: 261 bp

>AT168997 NZ_CP076268:c3773054-3772695 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A7G4RL39

Antitoxin

• AlphaFold DB: A0A7G4RL40