Detailed information of TA system TA168996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3676856..3678393
• Replicon: chromosome
• Accession: NZ_CP059576
• Organism: Legionella sp. PC997

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HBNCFIEN_RS16105
• Protein ID: WP_182392067.1
• Coordinates: 3677095..3678393 (+)
• Length: 433 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HBNCFIEN_RS16100
• Protein ID: WP_182392066.1
• Coordinates: 3676856..3677095 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBNCFIEN_RS16070	3672113..3672532	-	420	WP_182392060.1	hypothetical protein	-
HBNCFIEN_RS16075	3672732..3672962	-	231	WP_182392061.1	hypothetical protein	-
HBNCFIEN_RS16080	3673208..3673747	+	540	WP_182392062.1	hypothetical protein	-
HBNCFIEN_RS16085	3674287..3674481	+	195	WP_182392063.1	hypothetical protein	-
HBNCFIEN_RS16090	3674471..3675163	+	693	WP_182392064.1	HEPN domain-containing protein	-
HBNCFIEN_RS16095	3675589..3676437	-	849	WP_182392065.1	hypothetical protein	-
HBNCFIEN_RS16100	3676856..3677095	+	240	WP_182392066.1	helix-turn-helix domain-containing protein	Antitoxin
HBNCFIEN_RS16105	3677095..3678393	+	1299	WP_182392067.1	type II toxin-antitoxin system HipA family toxin	Toxin
HBNCFIEN_RS16110	3678476..3678955	-	480	WP_182392068.1	hypothetical protein	-
HBNCFIEN_RS16115	3679083..3679763	-	681	WP_182392069.1	hypothetical protein	-
HBNCFIEN_RS16120	3680283..3681374	+	1092	WP_182392070.1	DUF2263 domain-containing protein	-
HBNCFIEN_RS16125	3681543..3682568	+	1026	WP_182392071.1	succinylglutamate desuccinylase/aspartoacylase family protein	-
HBNCFIEN_RS16130	3682574..3683260	-	687	WP_182392072.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (148-398)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 433 a.a.
• Molecular weight: 49432.69 Da
• Isoelectric Point: 8.2954

>T168996 WP_182392067.1 NZ_CP059576:3677095-3678393 [Legionella sp. PC997]
MRKLYILMNNELVGELTQDNAGGLSFFYVEDWISKPHSRPVSLSLPLVNQKFSGEVVYNFFDNLLPDNPQIRARIQARFQ
AKSSRPFDLLALIGSDCVGAIQIVERIPQTQRLSIESHVVSEAEIARTLRSYQNSPLGMDKEDDAFRISLAGAQEKTAFL
YYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLDVAKSKILQFEDEKVLVVERFDRKWSRD
NRYVLRLPQEDLCQALGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQPQG
GYQLTPLYDIISAYPIVEQKKLSFKKLKMAMAIYGKNSHYKIDGIQKRHFLETAKYVQYSKIKAAQIFEQVIDRIDSAID
EVSTQLPNHFPQKIADPIFKGMRAFKERIIST

Nucleotide

• Length: 1299 bp

>T168996 NZ_CP076268:3444707-3445171 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9061.38 Da
• Isoelectric Point: 7.0116

>AT168996 WP_182392066.1 NZ_CP059576:3676856-3677095 [Legionella sp. PC997]
MIIRSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTVSKFENSPDNSRIDTLFRILSALNLNISLVDKGQKTHEEIEW

Nucleotide

• Length: 240 bp

>AT168996 NZ_CP076268:3444372-3444707 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA