Detailed information of TA system TA168984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1397065..1398746
• Replicon: chromosome
• Accession: NZ_CP059569
• Organism: Kingella oralis strain DSM 18271

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: C4GFD2
• Locus tag: H3L93_RS07545
• Protein ID: WP_003794581.1
• Coordinates: 1397065..1398408 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H3L93_RS07550
• Protein ID: WP_003794579.1
• Coordinates: 1398405..1398746 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3L93_RS07510	1392073..1393335	-	1263	WP_003794597.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
H3L93_RS07515	1393354..1393539	-	186	WP_003794595.1	hypothetical protein	-
H3L93_RS07520	1393572..1394414	-	843	WP_003794594.1	helix-turn-helix domain-containing protein	-
H3L93_RS07525	1394414..1395214	-	801	WP_155803112.1	type IV pilus biogenesis/stability protein PilW	-
H3L93_RS07530	1395204..1396304	-	1101	WP_003794590.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
H3L93_RS07535	1396393..1396581	-	189	WP_155802992.1	hypothetical protein	-
H3L93_RS07540	1396566..1396928	-	363	WP_003794585.1	Mth938-like domain-containing protein	-
H3L93_RS07545	1397065..1398408	+	1344	WP_003794581.1	type II toxin-antitoxin system HipA family toxin	Toxin
H3L93_RS07550	1398405..1398746	+	342	WP_003794579.1	helix-turn-helix domain-containing protein	Antitoxin
H3L93_RS07555	1398972..1400278	+	1307	Protein_1479	homoserine dehydrogenase	-
H3L93_RS07560	1400696..1401505	+	810	WP_003794570.1	NUDIX domain-containing protein	-
H3L93_RS07565	1401577..1401936	+	360	WP_003794569.1	hypothetical protein	-
H3L93_RS07570	1401997..1402614	+	618	WP_003794566.1	riboflavin synthase	-
H3L93_RS07575	1402786..1402986	-	201	WP_003794565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-394)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-100)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 50438.55 Da
• Isoelectric Point: 5.2749

>T168984 WP_003794581.1 NZ_CP059569:1397065-1398408 [Kingella oralis]
MQPENLTLQIFHHQQWHDAAELSFDASFVCTHLDYLNDYAFCQDENGDFLCWEKDDSYALGLNYPVNIMGYQNQPFTLLD
DIIPAGASRRYWVQHLGIARQPENQQNYTLLQRGTIAPIGNVRIKQAVPHEAATRHFQAAEAINRQADFLEYAQEHGAIA
GGATGAGGEAPKLLLRQTPSGEVWIDNQQDGKSADAYYLVKYPRNQRTPIDCDILRAEFYFYHELAALGFATINTRQMKL
EEGEHYPSLWLPRFDVEQAQGQTVRLAMESVYSLLERGAGSYLNHGETLRAIVRKIQHSHLVQAQGFQLDAGAWISQWLQ
RDLLNIAFGNSDNHGRNTAFIRRAGSIEFAPVYDFAPMKADPEGIIRSTTWGAPLENGGEYDFSAIAQSFADLADPDALL
AELQSTAEKLLDLKHRLHQRGVPSRILEMAGLGYDYLPEKLKRWGLL

Nucleotide

• Length: 1344 bp

>T168984 NZ_CP076268:c1337970-1337863 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12725.97 Da
• Isoelectric Point: 11.4481

>AT168984 WP_003794579.1 NZ_CP059569:1398405-1398746 [Kingella oralis]
MTTPTPPTPKQPRATKNKTTLSAQQRSAFRNQILRELWQGKLTQGQALKRLRIEMLGLKQAQFAKLVGISLPSLSNVEND
RVVSVELINKAFKPFGVALSLVPMRFDEMEAMF

Nucleotide

• Length: 342 bp

>AT168984 NZ_CP076268:1338018-1338084 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: C4GFD2