Detailed information of TA system TA168927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 633202..633826
• Replicon: chromosome
• Accession: NZ_CP059488
• Organism: Acidithiobacillus ferrivorans strain XJFY6S-08

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H2515_RS03180
• Protein ID: WP_085537859.1
• Coordinates: 633422..633826 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1B9BXH2
• Locus tag: H2515_RS03175
• Protein ID: WP_065413603.1
• Coordinates: 633202..633432 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H2515_RS03155	628261..630381	+	2121	WP_198661396.1	FAD-dependent oxidoreductase	-
H2515_RS03160	630710..631000	+	291	WP_198660901.1	hypothetical protein	-
H2515_RS03165	631538..631714	+	177	WP_176712600.1	hypothetical protein	-
H2515_RS03170	632397..632795	+	399	WP_198661397.1	GFA family protein	-
H2515_RS03175	633202..633432	+	231	WP_065413603.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H2515_RS03180	633422..633826	+	405	WP_085537859.1	PIN domain-containing protein	Toxin
H2515_RS03185	633994..634587	+	594	Protein_622	EAL domain-containing protein	-
H2515_RS03190	634723..635004	-	282	WP_065413606.1	EAL domain-containing protein	-
H2515_RS03195	635001..637478	-	2478	WP_198660902.1	DUF2309 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15112.48 Da
• Isoelectric Point: 5.1221

>T168927 WP_085537859.1 NZ_CP059488:633422-633826 [Acidithiobacillus ferrivorans]
MTSDKAFFDTNVLLYLLSEDTVKADRAESVIVQGGIISAQVLNEFASVARRKMAMSLPEVREVLTPLYRLCEVTSVTLDT
HIQGIQIAERYQYNIWDAMIVASALMAHCVTLYTEDMHHGQILEGRLHIVNPFL

Nucleotide

• Length: 405 bp

>T168927 NZ_CP076259:c3786876-3786646 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8688.96 Da
• Isoelectric Point: 8.7087

>AT168927 WP_065413603.1 NZ_CP059488:633202-633432 [Acidithiobacillus ferrivorans]
MQVSKWGNSLAVRLPAAVVAALGLKEGDNIEIEVLGEKQFQIQRTLQNAELLARLRRYRGRLPADFRFDRLEAHDE

Nucleotide

• Length: 231 bp

>AT168927 NZ_CP076259:c3786649-3786290 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1B9BXH2