Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 42625..43268 Replicon plasmid pIB2020_IncFIB
Accession NZ_CP059483
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name vagD Uniprot ID A0A330DBA3
Locus tag H1R18_RS25385 Protein ID WP_024553480.1
Coordinates 42625..43041 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A330D345
Locus tag H1R18_RS25390 Protein ID WP_024553479.1
Coordinates 43038..43268 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS25360 37730..37942 + 213 Protein_47 IS5/IS1182 family transposase -
H1R18_RS25365 38089..38679 - 591 WP_111969885.1 FadR family transcriptional regulator -
H1R18_RS25370 38731..39954 - 1224 WP_048998168.1 OprD family outer membrane porin -
H1R18_RS25375 40228..41208 - 981 WP_039025567.1 IS5-like element ISKpn26 family transposase -
H1R18_RS25380 41533..42501 - 969 WP_074181092.1 IS5 family transposase -
H1R18_RS25385 42625..43041 - 417 WP_024553480.1 type II toxin-antitoxin system VapC family toxin Toxin
H1R18_RS25390 43038..43268 - 231 WP_024553479.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H1R18_RS25400 45126..45452 - 327 WP_071789783.1 four-helix bundle copper-binding protein -
H1R18_RS25405 45642..45794 - 153 Protein_56 VapC toxin family PIN domain ribonuclease -
H1R18_RS25410 45812..45976 - 165 Protein_57 IS110 family transposase -
H1R18_RS25415 46039..47186 + 1148 WP_087794475.1 IS3 family transposase -
H1R18_RS25420 47207..47383 + 177 Protein_59 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..85067 85067
- inside IScluster/Tn - - 25671..47186 21515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15063.53 Da        Isoelectric Point: 7.7814

>T168921 WP_024553480.1 NZ_CP059483:c43041-42625 [Enterobacter kobei]
VNKIYMLDTCICSFIMREQPEAVLKRLEQAVLRNHRIVVSAITYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFARVPGLILEDWVN

Download         Length: 417 bp

>T168921 NZ_CP076259:c2262158-2261907 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8663.74 Da        Isoelectric Point: 4.3654

>AT168921 WP_024553479.1 NZ_CP059483:c43268-43038 [Enterobacter kobei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFMAEREDVVTDEGRVDL

Download         Length: 231 bp

>AT168921 NZ_CP076259:c2261910-2261656 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330DBA3

Antitoxin

Source ID Structure
AlphaFold DB A0A330D345

References