Detailed information of TA system TA168916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3656843..3657516
• Replicon: chromosome
• Accession: NZ_CP059481
• Organism: Enterobacter kobei strain IB2020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W0G4A4
• Locus tag: H1R18_RS18630
• Protein ID: WP_014882935.1
• Coordinates: 3657124..3657516 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H1R18_RS18625
• Protein ID: WP_050863273.1
• Coordinates: 3656843..3657127 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1R18_RS18590	3652395..3652955	+	561	WP_050863277.1	hypothetical protein	-
H1R18_RS18595	3652952..3653308	+	357	WP_050863276.1	hypothetical protein	-
H1R18_RS18600	3653308..3653808	+	501	WP_050863275.1	type VI secretion system tube protein Hcp	-
H1R18_RS18605	3653896..3654350	-	455	Protein_3450	hypothetical protein	-
H1R18_RS18610	3654744..3655100	+	357	WP_127353133.1	hypothetical protein	-
H1R18_RS18615	3655185..3655565	+	381	WP_182115512.1	hypothetical protein	-
H1R18_RS18620	3655559..3656256	-	698	Protein_3453	IS1 family transposase	-
H1R18_RS18625	3656843..3657127	-	285	WP_050863273.1	helix-turn-helix domain-containing protein	Antitoxin
H1R18_RS18630	3657124..3657516	-	393	WP_014882935.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H1R18_RS18635	3657653..3658873	-	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
H1R18_RS18640	3658977..3661010	-	2034	WP_023336880.1	choline BCCT transporter BetT	-
H1R18_RS18645	3661139..3661726	+	588	WP_014882933.1	transcriptional regulator BetI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3655559..3658873	3314

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (32-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-71)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14530.75 Da
• Isoelectric Point: 9.8533

>T168916 WP_014882935.1 NZ_CP059481:c3657516-3657124 [Enterobacter kobei]
MSHAIEFIETSLFTRQIKSIATDDELKDLQKELIARPDKGDLIQHTGGLRKIRLAAGSKGKRGGARVIYFLATEEVIYFI
MAYLKSTRDSLTEAEKAELKKLTSVLKNTTSYAINGRCTMYSIQAEGGRQ

Nucleotide

• Length: 393 bp

>T168916 NZ_CP076259:c1505858-1505664 [Escherichia coli]
TTGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGACATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCGCTCGAAAGATGGAATAAACGAA
CCACTGAAAATATTAATGGAGGCGTTCATGTATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10570.04 Da
• Isoelectric Point: 9.5343

>AT168916 WP_050863273.1 NZ_CP059481:c3657127-3656843 [Enterobacter kobei]
MSIFDELKSSLEEAVEIHSGRKAASRVTRYEVADVRAIREQLNITQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLYV
IQKDPAFYRALAEQ

Nucleotide

• Length: 285 bp

>AT168916 NZ_CP076259:1505487-1505665 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATTGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A2W0G4A4