Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 114925..116493 Replicon plasmid pIB2020_IncA
Accession NZ_CP059480
Organism Enterobacter kobei strain IB2020

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag H1R18_RS00645 Protein ID WP_024134513.1
Coordinates 114925..116232 (-) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag H1R18_RS00650 Protein ID WP_039272366.1
Coordinates 116242..116493 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1R18_RS00620 110107..111372 - 1266 Protein_123 DUF2235 domain-containing protein -
H1R18_RS00625 111701..112357 - 657 WP_012537714.1 quinolone resistance pentapeptide repeat protein QnrS2 -
H1R18_RS00630 112763..113122 - 360 WP_172693129.1 PAAR domain-containing protein -
H1R18_RS00635 113321..114259 + 939 WP_044596315.1 hypothetical protein -
H1R18_RS00640 114434..114928 - 495 WP_039272364.1 hypothetical protein -
H1R18_RS00645 114925..116232 - 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
H1R18_RS00650 116242..116493 - 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
H1R18_RS00655 116858..117862 - 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
H1R18_RS00660 117865..118428 - 564 WP_001132404.1 hypothetical protein -
H1R18_RS00665 118447..118728 - 282 WP_000575656.1 hypothetical protein -
H1R18_RS00670 118989..119591 + 603 WP_039272372.1 hypothetical protein -
H1R18_RS00675 119607..120059 - 453 WP_001553092.1 hypothetical protein -
H1R18_RS00680 120115..120840 - 726 WP_001553093.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / catB2 / ant(3'')-Ia / aph(3')-XV / blaVIM-1 / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / qnrS2 - 1..165722 165722


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T168904 WP_024134513.1 NZ_CP059480:c116232-114925 [Enterobacter kobei]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T168904 NZ_CP076257:c1287632-1287336 [Xanthomonas translucens pv. graminis]
GTGATCGAACTCAAGCAGACCGAGACGTTCCGCAAGTGGCGCACGAAGCTGAAGGACGAGCGCGCTCGCACTGCGATTGC
CTTGCGCTTGACCCGACTTGCCTATGGCCATGCCGGCGATACGGAAGCTGTCGGGCAAGGCATCAGCGAGCTGCGTATCC
ACCATGGCCCCGGATATCGGGTGTATTTCCAGCGGCGCGGCGACACGATCATCGTCTTGCTCTGCGGTGGCGACAAAAGC
TCGCAGGCAAAGGACATCAAGACAGCCAAACGGCTTGCCGACGAATGGAGCGAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT168904 WP_039272366.1 NZ_CP059480:c116493-116242 [Enterobacter kobei]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT168904 NZ_CP076257:c1287333-1287040 [Xanthomonas translucens pv. graminis]
ATGACCGAAAACCTGACCACCTACGATCCTGCCGAGGATCTGAACTCCGACCAGGCGATTGCCGATTTCATGGCGGGCGC
CTTTGCGACCAACGACACCGGCTTCATCGCCCATGCCCTCGGCATCGTCGCCCGTGCCAAGGGCATGACCCAGATCGCAC
GACAAACCGGCCTGTCCCGGGAGCAGCTCTACCGCTCCTTCAGCGAGGACGGAAATCCGACGCTGCGCACCACCCTGGCC
GTCATGAAGGCGCTCGGGATCGAACTGACGGCCAAAGCCGCCGTCACGCATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References