Detailed information of TA system TA168598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1470067..1470803
• Replicon: chromosome
• Accession: NZ_CP059312
• Organism: Klebsiella pneumoniae strain Kp46596

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A4S4Y1H2
• Locus tag: H1D28_RS09930
• Protein ID: WP_032433360.1
• Coordinates: 1470321..1470803 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H1D28_RS09925
• Protein ID: WP_003026799.1
• Coordinates: 1470067..1470333 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H1D28_RS09900	1465713..1466852	+	1140	WP_032433368.1	mannitol dehydrogenase	-
H1D28_RS09905	1466881..1467543	+	663	WP_032433366.1	dihydroxyacetone kinase subunit L	-
H1D28_RS09910	1467527..1468531	+	1005	WP_032433364.1	dihydroxyacetone kinase subunit DhaK	-
H1D28_RS09915	1468549..1469181	+	633	WP_032433362.1	3-hexulose-6-phosphate synthase	-
H1D28_RS09920	1469191..1469754	+	564	WP_032433361.1	6-phospho-3-hexuloisomerase	-
H1D28_RS09925	1470067..1470333	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H1D28_RS09930	1470321..1470803	+	483	WP_032433360.1	GNAT family N-acetyltransferase	Toxin
H1D28_RS09935	1471003..1472406	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
H1D28_RS09940	1472435..1473067	-	633	WP_001567369.1	hypothetical protein	-
H1D28_RS09945	1473447..1474046	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
H1D28_RS09950	1474259..1475203	-	945	WP_077254249.1	fimbrial protein	-
H1D28_RS09955	1475215..1475793	-	579	WP_032432061.1	type 1 fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1440290..1484028	43738
	-	flank	IS/Tn	-	-	1471003..1472406	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17421.02 Da
• Isoelectric Point: 9.6047

>T168598 WP_032433360.1 NZ_CP059312:1470321-1470803 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T168598 NZ_CP076123:4900105-4900482 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT168598 WP_003026799.1 NZ_CP059312:1470067-1470333 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT168598 NZ_CP076123:4899647-4900015 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A4S4Y1H2

Antitoxin

• AlphaFold DB: A0A1Q8YL66