Detailed information of TA system TA168565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 194258..194994
• Replicon: plasmid pER17974.3
• Accession: NZ_CP059296
• Organism: Klebsiella pneumoniae strain ER17974.3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: H0490_RS26925
• Protein ID: WP_004098919.1
• Coordinates: 194512..194994 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: H0490_RS26920
• Protein ID: WP_004213599.1
• Coordinates: 194258..194524 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0490_RS26895 (H0490_26850)	189490..190047	+	558	WP_004213590.1	recombinase family protein	-
H0490_RS26900 (H0490_26855)	190050..193019	+	2970	WP_004213592.1	Tn3 family transposase	-
H0490_RS26905 (H0490_26860)	193061..193555	+	495	WP_004213594.1	hypothetical protein	-
H0490_RS26910 (H0490_26865)	193616..193819	+	204	WP_004213596.1	HHA domain-containing protein	-
H0490_RS26915 (H0490_26870)	193833..194063	+	231	WP_004213598.1	hypothetical protein	-
H0490_RS26920 (H0490_26875)	194258..194524	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
H0490_RS26925 (H0490_26880)	194512..194994	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
H0490_RS26930 (H0490_26885)	195415..195693	+	279	Protein_215	transposase	-
H0490_RS26935 (H0490_26890)	195740..196546	-	807	WP_220014942.1	M14 family metallocarboxypeptidase	-
H0490_RS26940 (H0490_26895)	196539..197300	-	762	Protein_217	threonine--tRNA ligase	-
H0490_RS27405	197340..197540	-	201	Protein_218	TGS domain-containing protein	-
H0490_RS26945 (H0490_26900)	197991..198815	+	825	WP_004214540.1	DUF1460 domain-containing protein	-
H0490_RS26950 (H0490_26905)	198832..199698	-	867	WP_011251271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..234967	234967
	-	inside	IScluster/Tn	-	-	189490..195726	6236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T168565 WP_004098919.1 NZ_CP059296:194512-194994 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T168565 NZ_CP076115:c1435080-1434895 [Ralstonia solanacearum]
ATGCAATCTGCACAGCTAATCCGGATGCTCGAAGATGATGGATGGCAGCTGGTGCGCGTCAACGGCAGTCACCATCATTA
CAAGCACCCCGAAAAACCGGGCCTGCTGACGGTGCCTCACCCAAAGAAGGACCTGCCAATCGGGACAGTTCGTAGCATCC
AGAAGAAAGCCGCCGGCCTGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT168565 WP_004213599.1 NZ_CP059296:194258-194524 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT168565 NZ_CP076115:c1434825-1434424 [Ralstonia solanacearum]
ATGCAATTCCCCATCGCTATTCACAAGGATGAGGGGAGCGTCTATGGCGTAACGGTGCCGGATATCCCCGGCGTCCATTC
GTGGGGCGATACCATCGATGACGCTGTCCGCAACACGCGGGAGGCCATCGCGGGCCATCTACAGACCCTGGTAGAGCTTG
GCGAAGACGTAGACGTTACCTGCTCGACGATCGAAGACCTGACAAAGCAAGACGAATATGCTGGCGCTGTTTGGGCGCTT
ATTGATGTCGATGCAGAGGAGCTTGACCGCACCCCCGAACGCATCAATGTGTCGCTCCCCCGTTTTGTGCTCAAGAAGCT
GGATCGATTCGCTGAAAACCGGCACGAGACCCGAAGCGGGCTCTTAGCTCGCGCAGCCATGGAGCTAATGGCCCACGGCT
AA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A7D3T1D0