Detailed information of TA system TA168486

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3664336..3665167
• Replicon: chromosome
• Accession: NZ_CP059281
• Organism: Escherichia coli strain C9

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: H0272_RS17885
• Protein ID: WP_000854814.1
• Coordinates: 3664793..3665167 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A641DSP2
• Locus tag: H0272_RS17880
• Protein ID: WP_001546021.1
• Coordinates: 3664336..3664704 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0272_RS17845	3659393..3660463	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
H0272_RS17850	3660599..3661276	+	678	WP_001362823.1	hypothetical protein	-
H0272_RS17855	3661292..3661702	+	411	WP_000846704.1	hypothetical protein	-
H0272_RS17860	3661923..3662744	+	822	WP_001234710.1	DUF945 domain-containing protein	-
H0272_RS17865	3663007..3663480	+	474	WP_001385393.1	antirestriction protein	-
H0272_RS17870	3663496..3663972	+	477	WP_001186773.1	RadC family protein	-
H0272_RS17875	3664035..3664256	+	222	WP_000692323.1	DUF987 domain-containing protein	-
H0272_RS17880	3664336..3664704	+	369	WP_001546021.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
H0272_RS17885	3664793..3665167	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
H0272_RS17890	3665164..3665358	+	195	WP_000988601.1	hypothetical protein	-
H0272_RS17895	3665371..3665484	+	114	WP_001161660.1	DUF957 domain-containing protein	-
H0272_RS17900	3665847..3666593	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H0272_RS17905	3666608..3668149	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H0272_RS17910	3668360..3668506	-	147	Protein_3493	transposase domain-containing protein	-
H0272_RS17915	3668560..3668742	+	183	WP_001016348.1	hypothetical protein	-
H0272_RS17920	3668843..3669172	-	330	WP_000450409.1	DUF496 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T168486 WP_000854814.1 NZ_CP059281:3664793-3665167 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T168486 NZ_CP076027:c842333-842145 [Staphylococcus sp. MZ7]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13607.47 Da
• Isoelectric Point: 6.3139

>AT168486 WP_001546021.1 NZ_CP059281:3664336-3664704 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGLFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT168486 NZ_CP076027:c842622-842362 [Staphylococcus sp. MZ7]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A641DSP2