Detailed information of TA system TA168155

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 196243..196669
• Replicon: plasmid pSY3626C1_315k
• Accession: NZ_CP059044
• Organism: Escherichia coli strain SY3626C1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HZ987_RS23640
• Protein ID: WP_001312861.1
• Coordinates: 196243..196401 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	196445..196669 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZ987_RS23615	191612..192301	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
HZ987_RS23620	192488..192871	-	384	WP_001151524.1	relaxosome protein TraM	-
HZ987_RS23625	193192..193794	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HZ987_RS23630	194091..194912	-	822	WP_072795862.1	DUF945 domain-containing protein	-
HZ987_RS23635	195034..195321	-	288	WP_000107535.1	hypothetical protein	-
HZ987_RS23640	196243..196401	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	196445..196669	-	225	NuclAT_0	-	Antitoxin
-	196445..196669	-	225	NuclAT_0	-	Antitoxin
-	196445..196669	-	225	NuclAT_0	-	Antitoxin
-	196445..196669	-	225	NuclAT_0	-	Antitoxin
HZ987_RS23645	196481..196669	+	189	WP_001299721.1	hypothetical protein	-
HZ987_RS23650	196681..197400	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HZ987_RS23655	197397..197831	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HZ987_RS23660	197886..199844	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
HZ987_RS23665	199909..200142	-	234	WP_000005987.1	DUF905 family protein	-
HZ987_RS23670	200199..200738	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
HZ987_RS23675	200764..200970	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(X4) / floR / qnrS1 / blaTEM-1B / ant(3'')-Ia / aac(3)-IId / lnu(F) / sul3 / aph(3')-Ia / sul2 / tet(M) / lnu(G)	-	1..315984	315984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T168155 WP_001312861.1 NZ_CP059044:c196401-196243 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T168155 NZ_CP075649:1477610-1478008 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT168155 NZ_CP059044:c196669-196445 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin