Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2805876..2806660 Replicon chromosome
Accession NZ_CP059003
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag H0E72_RS13655 Protein ID WP_000613626.1
Coordinates 2806166..2806660 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag H0E72_RS13650 Protein ID WP_001110446.1
Coordinates 2805876..2806169 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS13640 2801037..2801996 - 960 WP_000846337.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
H0E72_RS13645 2802569..2805742 + 3174 WP_075591672.1 ribonuclease E -
H0E72_RS13650 2805876..2806169 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
H0E72_RS13655 2806166..2806660 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
H0E72_RS13660 2806755..2807708 - 954 WP_001212763.1 flagellar hook-associated protein FlgL -
H0E72_RS13665 2807720..2809363 - 1644 WP_000096448.1 flagellar hook-associated protein FlgK -
H0E72_RS13670 2809429..2810370 - 942 WP_012311956.1 flagellar assembly peptidoglycan hydrolase FlgJ -
H0E72_RS13675 2810370..2811467 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T168089 WP_000613626.1 NZ_CP059003:2806166-2806660 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T168089 NZ_CP075632:1207578-1207685 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT168089 WP_001110446.1 NZ_CP059003:2805876-2806169 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT168089 NZ_CP075632:c1207531-1207465 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References