Detailed information of TA system TA167953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 185274..185881
• Replicon: plasmid plas4
• Accession: NZ_CP058895
• Organism: Enterococcus faecium strain ef332

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: HZU50_RS15240
• Protein ID: WP_002321032.1
• Coordinates: 185531..185881 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: HZU50_RS15235
• Protein ID: WP_002287514.1
• Coordinates: 185274..185537 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZU50_RS15195 (HZU50_15195)	180731..180991	+	261	WP_002296240.1	hypothetical protein	-
HZU50_RS15200 (HZU50_15200)	181432..181638	+	207	WP_002296239.1	hypothetical protein	-
HZU50_RS15205 (HZU50_15205)	181638..181889	+	252	WP_002296238.1	hypothetical protein	-
HZU50_RS15210 (HZU50_15210)	181904..182341	+	438	WP_002296237.1	hypothetical protein	-
HZU50_RS15215 (HZU50_15215)	182334..183041	+	708	WP_002353391.1	hypothetical protein	-
HZU50_RS15220 (HZU50_15220)	183206..183406	+	201	WP_002330566.1	hypothetical protein	-
HZU50_RS15225 (HZU50_15225)	183422..183601	+	180	WP_002305761.1	hypothetical protein	-
HZU50_RS15230 (HZU50_15230)	183825..184997	+	1173	WP_002323245.1	IS256-like element IS1542 family transposase	-
HZU50_RS15235 (HZU50_15235)	185274..185537	+	264	WP_002287514.1	hypothetical protein	Antitoxin
HZU50_RS15240 (HZU50_15240)	185531..185881	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HZU50_RS15245 (HZU50_15245)	185905..186519	-	615	WP_010729795.1	recombinase family protein	-
HZU50_RS15250 (HZU50_15250)	186833..187255	+	423	WP_002303116.1	hypothetical protein	-
HZU50_RS15255 (HZU50_15255)	187265..187468	+	204	WP_002299573.1	hypothetical protein	-
HZU50_RS15260 (HZU50_15260)	187679..188263	+	585	WP_002299575.1	recombinase family protein	-
HZU50_RS15265 (HZU50_15265)	188435..189115	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-
HZU50_RS15270 (HZU50_15270)	189177..189923	+	747	WP_238786685.1	hypothetical protein	-
HZU50_RS15275 (HZU50_15275)	190012..190398	+	387	WP_000391061.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aac(6')-aph(2'')	-	1..203651	203651
	-	inside	IScluster/Tn	aac(6')-aph(2'')	-	183825..198893	15068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T167953 WP_002321032.1 NZ_CP058895:185531-185881 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T167953 NZ_CP075487:2596315-2596422 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCATCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT167953 WP_002287514.1 NZ_CP058895:185274-185537 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT167953 NZ_CP075487:c2596262-2596201 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0