Detailed information of TA system TA167921

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37420..37684
• Replicon: plasmid unnamed3
• Accession: NZ_CP058882
• Organism: Escherichia coli strain STLEFF_29

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: HZT24_RS24950
• Protein ID: WP_001387489.1
• Coordinates: 37532..37684 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	37420..37480 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT24_RS24935 (33522)	33522..34592	-	1071	WP_011264050.1	IncI1-type conjugal transfer protein TrbB	-
HZT24_RS24940 (34611)	34611..35819	-	1209	WP_011264049.1	IncI1-type conjugal transfer protein TrbA	-
- (35999)	35999..36056	-	58	NuclAT_1	-	-
- (35999)	35999..36056	-	58	NuclAT_1	-	-
- (35999)	35999..36056	-	58	NuclAT_1	-	-
- (35999)	35999..36056	-	58	NuclAT_1	-	-
HZT24_RS24945 (36126)	36126..37211	-	1086	WP_074446708.1	protein finQ	-
- (37420)	37420..37480	-	61	NuclAT_0	-	Antitoxin
- (37420)	37420..37480	-	61	NuclAT_0	-	Antitoxin
- (37420)	37420..37480	-	61	NuclAT_0	-	Antitoxin
- (37420)	37420..37480	-	61	NuclAT_0	-	Antitoxin
HZT24_RS24950 (37532)	37532..37684	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
HZT24_RS24955 (37756)	37756..38007	-	252	WP_001291964.1	hypothetical protein	-
HZT24_RS25625 (38508)	38508..38603	+	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
HZT24_RS24965 (38668)	38668..38844	-	177	WP_001054898.1	hypothetical protein	-
HZT24_RS24970 (39027)	39027..39236	-	210	WP_001140543.1	hemolysin expression modulator Hha	-
HZT24_RS24975 (39334)	39334..39948	-	615	WP_000578648.1	plasmid IncI1-type surface exclusion protein ExcA	-
HZT24_RS24980 (40024)	40024..42192	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82730	82730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T167921 WP_001387489.1 NZ_CP058882:37532-37684 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T167921 NZ_CP075484:717305-717682 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTATGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT167921 NZ_CP058882:c37480-37420 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin