Detailed information of TA system TA167737

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1918228..1918599
• Replicon: chromosome
• Accession: NZ_CP058853
• Organism: Shigella flexneri strain STLEFF_38

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A7W3G0W6
• Locus tag: HZT29_RS09245
• Protein ID: WP_072130784.1
• Coordinates: 1918405..1918599 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1918228..1918406 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZT29_RS09220 (1914486)	1914486..1915421	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HZT29_RS09225 (1915473)	1915473..1916708	-	1236	WP_000040843.1	site-specific integrase	-
HZT29_RS09230 (1916710)	1916710..1916925	-	216	WP_000079604.1	excisionase XisR	-
HZT29_RS09235 (1917102)	1917102..1918082	-	981	WP_000019402.1	IS5-like element IS5 family transposase	-
- (1918228)	1918228..1918406	+	179	NuclAT_0	-	Antitoxin
- (1918228)	1918228..1918406	+	179	NuclAT_0	-	Antitoxin
- (1918228)	1918228..1918406	+	179	NuclAT_0	-	Antitoxin
- (1918228)	1918228..1918406	+	179	NuclAT_0	-	Antitoxin
HZT29_RS09240 (1918224)	1918224..1918412	-	189	WP_001302840.1	DUF1187 family protein	-
HZT29_RS09245 (1918405)	1918405..1918599	-	195	WP_072130784.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HZT29_RS09250 (1918656)	1918656..1919489	-	834	WP_000166322.1	recombination protein RecT	-
HZT29_RS09255 (1919482)	1919482..1922082	-	2601	WP_239637371.1	exodeoxyribonuclease VIII	-
HZT29_RS09260 (1922184)	1922184..1922459	-	276	WP_000632297.1	protein RacC	-
HZT29_RS09265 (1922534)	1922534..1922704	-	171	WP_001352098.1	YdaE family protein	-
HZT29_RS09270 (1922704)	1922704..1922925	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	-	1915473..1973564	58091
	-	flank	IS/Tn	-	-	1917102..1918082	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7135.00 Da
• Isoelectric Point: 8.0115

>T167737 WP_072130784.1 NZ_CP058853:c1918599-1918405 [Shigella flexneri]
MRYDDVKPCPFCGCPSVTVKDISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTENINGGVHV

Nucleotide

• Length: 195 bp

>T167737 NZ_CP075374:1996024-1996275 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT167737 NZ_CP058853:1918228-1918406 [Shigella flexneri]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATTGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7W3G0W6

Antitoxin