Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17636..18279 Replicon plasmid unnamed1
Accession NZ_CP058847
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HZT27_RS23235 Protein ID WP_001044768.1
Coordinates 17863..18279 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HZT27_RS23230 Protein ID WP_001261287.1
Coordinates 17636..17866 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS23210 (HZT27_22975) 12762..13850 + 1089 WP_000952217.1 transcriptional repressor PifC -
HZT27_RS23215 (HZT27_22980) 13852..16077 + 2226 WP_000698737.1 P-loop NTPase fold protein -
HZT27_RS23220 (HZT27_22985) 16127..17026 - 900 WP_000963206.1 nucleotide-binding protein -
HZT27_RS23225 (HZT27_22990) 17016..17306 - 291 WP_000111771.1 hypothetical protein -
HZT27_RS23230 (HZT27_22995) 17636..17866 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HZT27_RS23235 (HZT27_23000) 17863..18279 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HZT27_RS23240 (HZT27_23005) 18441..20579 - 2139 WP_000350638.1 AAA family ATPase -
HZT27_RS23245 (HZT27_23010) 20933..21190 + 258 WP_000343085.1 hypothetical protein -
HZT27_RS23250 (HZT27_23015) 21190..21780 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA14 / aph(3')-Ia / blaCTX-M-55 / blaTEM-1B / mph(A) / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / fosA3 / sitABCD - 1..85837 85837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T167704 WP_001044768.1 NZ_CP058847:17863-18279 [Shigella flexneri]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T167704 NZ_CP075345:3638275-3638493 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGCCGTTGTCAGTCAATTGACACCCTCGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATTCCCACATCCGTGTGGAAATTTGTACGTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT167704 WP_001261287.1 NZ_CP058847:17636-17866 [Shigella flexneri]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT167704 NZ_CP075345:3637873-3638247 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCGCAAGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References