Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2570991..2571462 Replicon chromosome
Accession NZ_CP058846
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag HZT27_RS12665 Protein ID WP_001303511.1
Coordinates 2571184..2571462 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag HZT27_RS12660 Protein ID WP_001302048.1
Coordinates 2570991..2571182 (+) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS12605 (2566188) 2566188..2566547 - 360 WP_240766961.1 hypothetical protein -
HZT27_RS12610 (2566544) 2566544..2566720 - 177 WP_000753060.1 hypothetical protein -
HZT27_RS12615 (2566713) 2566713..2566895 - 183 WP_001224672.1 hypothetical protein -
HZT27_RS12620 (2566989) 2566989..2567345 - 357 WP_208311420.1 hypothetical protein -
HZT27_RS12625 (2567347) 2567347..2567910 - 564 WP_155104913.1 hypothetical protein -
HZT27_RS12630 (2567876) 2567876..2568175 - 300 WP_032201977.1 DUF4406 domain-containing protein -
HZT27_RS12635 (2568172) 2568172..2568594 - 423 WP_240766962.1 DUF977 family protein -
HZT27_RS12640 (2568635) 2568635..2569705 - 1071 WP_240766963.1 phage replisome organizer -
HZT27_RS12645 (2569777) 2569777..2570202 - 426 WP_000693883.1 toxin YdaT family protein -
HZT27_RS12650 (2570199) 2570199..2570501 - 303 WP_000172735.1 transcriptional regulator -
HZT27_RS12655 (2570599) 2570599..2570970 + 372 WP_001169685.1 hypothetical protein -
HZT27_RS12660 (2570991) 2570991..2571182 + 192 WP_001302048.1 hypothetical protein Antitoxin
HZT27_RS12665 (2571184) 2571184..2571462 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HZT27_RS12670 (2571754) 2571754..2571909 + 156 WP_240766964.1 DUF1391 family protein -
HZT27_RS24075 (2571911) 2571911..2572039 + 129 WP_021573425.1 protein YdfB -
HZT27_RS12675 (2572069) 2572069..2572287 + 219 WP_033560095.1 protein YdfC -
HZT27_RS12680 (2572853) 2572853..2573041 + 189 WP_000854559.1 cell division inhibition protein DicB -
HZT27_RS12685 (2573038) 2573038..2573229 + 192 WP_001083273.1 lysis protein YdfD -
HZT27_RS12690 (2573322) 2573322..2575793 + 2472 WP_240766965.1 exonuclease -
HZT27_RS12695 (2575852) 2575852..2576055 + 204 WP_000096344.1 DUF4224 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T167698 WP_001303511.1 NZ_CP058846:2571184-2571462 [Shigella flexneri]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T167698 NZ_CP075345:31714-32028 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCCCGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT167698 WP_001302048.1 NZ_CP058846:2570991-2571182 [Shigella flexneri]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT167698 NZ_CP075345:32025-32444 [Enterobacter kobei]
ATGATGATCGTAGCTGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGGCCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTGACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCTCGCTTTGGGGTATCGC
CTGCGCTGTTCATTGAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References