Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 473313..473889 Replicon chromosome
Accession NZ_CP058846
Organism Shigella flexneri strain STLEFF_35

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag HZT27_RS02205 Protein ID WP_001297643.1
Coordinates 473313..473600 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag HZT27_RS02210 Protein ID WP_000063156.1
Coordinates 473587..473889 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZT27_RS02185 (469566) 469566..470846 - 1281 WP_001037400.1 DUF445 domain-containing protein -
HZT27_RS02190 (470887) 470887..472119 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
HZT27_RS02195 (472265) 472265..472360 - 96 Protein_423 dienelactone hydrolase family protein -
HZT27_RS02200 (472417) 472417..473114 + 698 Protein_424 IS1 family transposase -
HZT27_RS02205 (473313) 473313..473600 + 288 WP_001297643.1 BrnT family toxin Toxin
HZT27_RS02210 (473587) 473587..473889 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
HZT27_RS02215 (473923) 473923..474879 - 957 WP_001297640.1 GTPase -
HZT27_RS02220 (474890) 474890..475093 - 204 WP_000467859.1 YbdD/YjiX family protein -
HZT27_RS02225 (475143) 475143..477293 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T167679 WP_001297643.1 NZ_CP058846:473313-473600 [Shigella flexneri]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T167679 NZ_CP075325:c4725254-4725102 [Xanthomonas citri pv. citri]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAATGCAGCGACGGTAAGTGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT167679 WP_000063156.1 NZ_CP058846:473587-473889 [Shigella flexneri]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT167679 NZ_CP075325:c4725027-4724893 [Xanthomonas citri pv. citri]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References