Detailed information of TA system TA167550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3783575..3784269
• Replicon: chromosome
• Accession: NZ_CP058796
• Organism: Shigella flexneri strain STLIN_8

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HZT12_RS18830
• Protein ID: WP_001263493.1
• Coordinates: 3783575..3783973 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HZT12_RS18835
• Protein ID: WP_000554757.1
• Coordinates: 3783976..3784269 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3779235)	3779235..3779315	-	81	NuclAT_11	-	-
- (3779235)	3779235..3779315	-	81	NuclAT_11	-	-
- (3779235)	3779235..3779315	-	81	NuclAT_11	-	-
- (3779235)	3779235..3779315	-	81	NuclAT_11	-	-
HZT12_RS18800 (3778575)	3778575..3779819	-	1245	WP_000189541.1	esterase FrsA	-
HZT12_RS18805 (3779911)	3779911..3780369	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HZT12_RS18810 (3780630)	3780630..3782087	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
HZT12_RS18815 (3782144)	3782144..3782665	-	522	Protein_3694	peptide chain release factor H	-
HZT12_RS18820 (3782664)	3782664..3782867	-	204	Protein_3695	RtcB family protein	-
HZT12_RS18825 (3783113)	3783113..3783565	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HZT12_RS18830 (3783575)	3783575..3783973	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HZT12_RS18835 (3783976)	3783976..3784269	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HZT12_RS18840 (3784321)	3784321..3785376	-	1056	WP_112034092.1	DNA polymerase IV	-
HZT12_RS18845 (3785447)	3785447..3786232	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HZT12_RS18850 (3786204)	3786204..3787916	+	1713	Protein_3701	flagellar biosynthesis protein FlhA	-
HZT12_RS18855 (3788132)	3788132..3788629	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T167550 WP_001263493.1 NZ_CP058796:c3783973-3783575 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T167550 NZ_CP075169:748566-748847 [Sodalis ligni]
ATGATTAAAAGTTTCAAGCACAAAGGAATGGAAAGGTTTTATAAAACGGGTTCTGCCGCCGGCATTCAAACTGCCCACAC
TTCGCGATTACGCGGGCAGTTGTTTGCCTTAAGCAAGGCTAAAAAGCCTTGTGATATGGGCGCTCCGGGATGGAATCTCC
ATCCCTTGAAAGGGAACGAGGCTGGCGTTTGGTCTGTGTCGGTTAACGGTGACTGGCGTATGACGTTTCGTTTTGATGCT
GATGGCGACGCCGTTCTCGTCGATTATCGCGACTATCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT167550 WP_000554757.1 NZ_CP058796:c3784269-3783976 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT167550 NZ_CP075169:748859-749146 [Sodalis ligni]
ATGCGTATGTTTAATCCGCCTCACCCCGGTGAGGTTTTGCGTGATTACCTGGAAGGGGTTAGCGTGACCGACGCCGCGGC
GTCGCTAAAGATAACGCGCACGCAACTGTCCCGTATCCTGAATGCACATGCGGCGATTACTGCTGACATGGCGTTGCGTT
TATCCAGTCTGCTTGGCACAAGTGCGGAAATGTGGGTGGATATGCAGTCTGAATATGAACTCTGGCAGGCGGCACAGAGG
CCGCGCCCGGTTATCGAACCGCTGCATAGACCGCACGTTCACGCCTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6