Detailed information of TA system TA167254

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2734351..2735191
• Replicon: chromosome
• Accession: NZ_CP058719
• Organism: Escherichia coli strain M159-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: HZS86_RS13225
• Protein ID: WP_000854686.1
• Coordinates: 2734808..2735191 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M0YJ34
• Locus tag: HZS86_RS13220
• Protein ID: WP_001360163.1
• Coordinates: 2734351..2734731 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS86_RS13195 (2731467)	2731467..2732285	+	819	WP_001234732.1	DUF932 domain-containing protein	-
HZS86_RS13200 (2732377)	2732377..2732862	+	486	WP_000214307.1	antirestriction protein	-
HZS86_RS13205 (2732878)	2732878..2733354	+	477	WP_001186726.1	RadC family protein	-
HZS86_RS13210 (2733417)	2733417..2733638	+	222	WP_000692298.1	DUF987 domain-containing protein	-
HZS86_RS13215 (2733657)	2733657..2734340	+	684	WP_000086769.1	hypothetical protein	-
HZS86_RS13220 (2734351)	2734351..2734731	+	381	WP_001360163.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZS86_RS13225 (2734808)	2734808..2735191	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
HZS86_RS13230 (2735188)	2735188..2735676	+	489	WP_001054233.1	DUF5983 family protein	-
HZS86_RS13235 (2735693)	2735693..2735890	+	198	WP_000839253.1	DUF957 domain-containing protein	-
HZS86_RS13240 (2735975)	2735975..2736817	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
HZS86_RS13250 (2737307)	2737307..2737525	-	219	WP_001040187.1	translation initiation factor IF-1	-
HZS86_RS13255 (2737810)	2737810..2738514	-	705	WP_001241677.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T167254 WP_000854686.1 NZ_CP058719:2734808-2735191 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T167254 NZ_CP075107:801052-801465 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13950.74 Da
• Isoelectric Point: 5.0823

>AT167254 WP_001360163.1 NZ_CP058719:2734351-2734731 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCIVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT167254 NZ_CP075107:800805-801071 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1M0YJ34