Detailed information of TA system TA167203

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22845..23271
• Replicon: plasmid pS163-2.3
• Accession: NZ_CP058712
• Organism: Escherichia coli strain S163-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HZS88_RS24405
• Protein ID: WP_001372321.1
• Coordinates: 22845..22970 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23047..23271 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS88_RS24360 (17892)	17892..18119	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
HZS88_RS24365 (18213)	18213..18899	-	687	WP_000332484.1	PAS domain-containing protein	-
HZS88_RS24370 (19090)	19090..19473	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HZS88_RS24375 (19750)	19750..20397	+	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
HZS88_RS24380 (20694)	20694..21515	-	822	WP_001234469.1	DUF932 domain-containing protein	-
HZS88_RS24385 (21636)	21636..21923	-	288	WP_000107544.1	hypothetical protein	-
HZS88_RS24390 (21948)	21948..22154	-	207	WP_000547968.1	hypothetical protein	-
HZS88_RS24395 (22224)	22224..22397	+	174	Protein_33	hypothetical protein	-
HZS88_RS24400 (22395)	22395..22625	-	231	WP_001426396.1	hypothetical protein	-
HZS88_RS24405 (22845)	22845..22970	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HZS88_RS24410 (22912)	22912..23061	-	150	Protein_36	plasmid maintenance protein Mok	-
- (23049)	23049..23114	+	66	NuclAT_0	-	-
- (23049)	23049..23114	+	66	NuclAT_0	-	-
- (23049)	23049..23114	-	66	NuclAT_1	-	-
- (23047)	23047..23271	+	225	NuclAT_0	-	-
- (23047)	23047..23271	-	225	NuclAT_0	-	Antitoxin
- (23047)	23047..23271	-	225	NuclAT_0	-	Antitoxin
- (23047)	23047..23271	-	225	NuclAT_0	-	Antitoxin
- (23047)	23047..23271	-	225	NuclAT_0	-	Antitoxin
HZS88_RS24415 (23083)	23083..23271	+	189	WP_001299721.1	hypothetical protein	-
HZS88_RS24420 (23240)	23240..24002	-	763	Protein_38	plasmid SOS inhibition protein A	-
HZS88_RS24425 (23999)	23999..24433	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HZS88_RS24430 (24488)	24488..26446	-	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
HZS88_RS24435 (26512)	26512..26745	-	234	WP_000005990.1	DUF905 family protein	-
HZS88_RS24440 (26808)	26808..27347	-	540	WP_001825195.1	single-stranded DNA-binding protein	-
HZS88_RS24445 (27373)	27373..27579	-	207	WP_000275858.1	hypothetical protein	-
HZS88_RS24450 (27820)	27820..28086	-	267	WP_072667006.1	hypothetical protein	-
HZS88_RS24455 (27990)	27990..28211	-	222	WP_042111886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T167203 WP_001372321.1 NZ_CP058712:c22970-22845 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T167203 NZ_CP075061:c2047017-2046910 [Escherichia coli]
ATGACGCTCGCGCAGTTTACCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT167203 NZ_CP058712:c23271-23047 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin