Detailed information of TA system TA167085

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66564..66818
• Replicon: plasmid pCTXM15_005008
• Accession: NZ_CP058663
• Organism: Escherichia coli strain 005008

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HZR83_RS24040
• Protein ID: WP_015387399.1
• Coordinates: 66564..66770 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	66757..66818 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR83_RS24010	61811..62722	-	912	WP_000440183.1	carbamate kinase	-
HZR83_RS24015	62733..63953	-	1221	WP_000410951.1	arginine deiminase	-
HZR83_RS24020	64846..65328	+	483	Protein_76	VENN motif pre-toxin domain-containing protein	-
HZR83_RS24025	65274..65720	-	447	Protein_77	incFII family plasmid replication initiator RepA	-
HZR83_RS24030	65713..65787	-	75	WP_032336874.1	RepA leader peptide Tap	-
HZR83_RS24035	66023..66280	-	258	WP_000083833.1	replication regulatory protein RepA	-
HZR83_RS24040	66564..66770	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66757..66818	+	62	NuclAT_2	-	Antitoxin
-	66757..66818	+	62	NuclAT_2	-	Antitoxin
-	66757..66818	+	62	NuclAT_2	-	Antitoxin
-	66757..66818	+	62	NuclAT_2	-	Antitoxin
HZR83_RS24045	66957..67139	-	183	WP_000968309.1	hypothetical protein	-
HZR83_RS24050	67240..67856	+	617	Protein_82	IS1 family transposase	-
HZR83_RS24055	67894..69465	-	1572	WP_023149734.1	IS66 family transposase	-
HZR83_RS24060	69485..69832	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HZR83_RS24065	69832..70509	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
HZR83_RS24070	70564..70653	+	90	Protein_86	IS1 family transposase	-
HZR83_RS24075	70954..71166	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / sitABCD	iutA / iucD / iucC / iucB / iucA	1..149236	149236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T167085 WP_015387399.1 NZ_CP058663:c66770-66564 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T167085 NZ_CP074677:3825612-3825830 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT167085 NZ_CP058663:66757-66818 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin