Detailed information of TA system TA167081

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63041..63305
• Replicon: plasmid pCMY42_005008
• Accession: NZ_CP058662
• Organism: Escherichia coli strain 005008

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: HZR83_RS23605
• Protein ID: WP_001387489.1
• Coordinates: 63041..63193 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	63245..63305 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR83_RS23570	58606..58722	+	117	Protein_68	IncI1-type conjugal transfer lipoprotein TraH	-
HZR83_RS23575	58721..60475	+	1755	Protein_69	DotA/TraY family protein	-
HZR83_RS23580	60546..61208	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
HZR83_RS23585	61280..61489	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HZR83_RS23590	61881..62057	+	177	WP_001054897.1	hypothetical protein	-
HZR83_RS23595	62122..62418	-	297	WP_011264046.1	hypothetical protein	-
HZR83_RS23600	62718..62969	+	252	WP_001291964.1	hypothetical protein	-
HZR83_RS23605	63041..63193	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	63245..63305	+	61	NuclAT_0	-	Antitoxin
-	63245..63305	+	61	NuclAT_0	-	Antitoxin
-	63245..63305	+	61	NuclAT_0	-	Antitoxin
-	63245..63305	+	61	NuclAT_0	-	Antitoxin
HZR83_RS23610	63508..63855	+	348	Protein_76	protein finQ	-
HZR83_RS23615	64041..65198	+	1158	Protein_77	IS1380-like element ISEc9 family transposase	-
HZR83_RS23620	65254..65951	+	698	Protein_78	IS1 family transposase	-
HZR83_RS23625	65965..66270	-	306	Protein_79	transcription termination factor NusG	-
HZR83_RS23630	66712..66999	-	288	WP_000074855.1	conjugal transfer protein TraA	-
HZR83_RS23635	66917..67180	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..68106	68106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T167081 WP_001387489.1 NZ_CP058662:c63193-63041 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T167081 NZ_CP074677:809375-809785 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT167081 NZ_CP058662:63245-63305 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin