Detailed information of TA system TA167075

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3593105..3593940
• Replicon: chromosome
• Accession: NZ_CP058661
• Organism: Escherichia coli strain 005008

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: HZR83_RS17310
• Protein ID: WP_000854760.1
• Coordinates: 3593563..3593940 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: HZR83_RS17305
• Protein ID: WP_001295723.1
• Coordinates: 3593105..3593473 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZR83_RS17265	3588170..3588334	-	165	WP_001128468.1	hypothetical protein	-
HZR83_RS17270	3588765..3589013	+	249	Protein_3360	transposase	-
HZR83_RS17275	3589023..3589655	-	633	Protein_3361	IS30-like element IS30 family transposase	-
HZR83_RS17280	3589711..3590408	+	698	Protein_3362	IS1 family transposase	-
HZR83_RS17285	3590527..3591345	+	819	WP_047149353.1	DUF945 domain-containing protein	-
HZR83_RS17290	3591687..3592160	+	474	WP_001387789.1	antirestriction protein	-
HZR83_RS17295	3592176..3592652	+	477	WP_001354275.1	RadC family protein	-
HZR83_RS17300	3592721..3592942	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HZR83_RS17305	3593105..3593473	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HZR83_RS17310	3593563..3593940	+	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
HZR83_RS17315	3593937..3594140	+	204	WP_000761705.1	hypothetical protein	-
HZR83_RS17320	3594153..3594266	+	114	WP_032140621.1	DUF957 domain-containing protein	-
HZR83_RS17325	3594425..3595453	+	1029	Protein_3371	IS110 family transposase	-
HZR83_RS17330	3595907..3596089	+	183	WP_024174802.1	ethanolamine utilization protein	-
HZR83_RS17335	3596190..3596519	-	330	WP_000450409.1	DUF496 family protein	-
HZR83_RS17340	3596691..3597749	-	1059	WP_001200895.1	FUSC family protein	-
HZR83_RS17345	3597947..3598420	-	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T167075 WP_000854760.1 NZ_CP058661:3593563-3593940 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T167075 NZ_CP074676:1020662-1020955 [Pseudomonas qingdaonensis]
GTGATCTATTCCATAGAACAGACTTTGGCATTCAGCGGCTGGCTTCTGGCGCTACGCGATGTGCGAGCGAGGATCGCCAT
TGGTCGACGCATCGAACGTGCGCTTGCGGGCAACTTGGGGGATACGAAGGCGGTTGGTGATGGTGTTTCAGAGATGCGCA
TCGATTCCGGCCCAGGCTATCGGGTGTACTTCACCGTGCGTAGCGGCGTAGCAATCTTGTTGCTGACAGGCGGGGACAAG
TCTTCTCAAGCAGCAGATATCCGGATGGCCAAGCGGTTGGCCAAAGAGGTTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT167075 WP_001295723.1 NZ_CP058661:3593105-3593473 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT167075 NZ_CP074676:1020958-1021257 [Pseudomonas qingdaonensis]
ATGAGCGTTACATTGATTCCATTCGACATGGCGGCACTGCTTGATAGTGATGAAGCCATCAGCGAGTACTTGTCTCAGGT
GCTGGCAGAGGGCGACAGCGACGAGCTTTTACGTGCAATAGGGTACGTGGCCAAAGCGCGCGGAATGTCGCTGGTTGCCA
AAGAGACTGGATTGGGAAGAGAGAGCCTTTACAAGGCCTTCGCGCCAGGCGCCAAGCCTCGTTTCGATACGGTGCTGAAG
GTGATGCATGCGCTGGGAATCGAACTCTGTGCGCGCTCGCATCGAGGGGCTTCGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3

Antitoxin

• AlphaFold DB: S1NM52