Detailed information of TA system TA166873

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4417..4686
• Replicon: plasmid pEC1-2
• Accession: NZ_CP058576
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HYH05_RS25265
• Protein ID: WP_001312861.1
• Coordinates: 4528..4686 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4417..4482 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS25230	1..1362	+	1362	WP_000170695.1	DUF3560 domain-containing protein	-
HYH05_RS25235	1409..1972	+	564	WP_001298559.1	class I SAM-dependent methyltransferase	-
HYH05_RS25240	2058..2531	-	474	WP_179226630.1	hypothetical protein	-
HYH05_RS25245	2833..3360	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HYH05_RS25250	3418..3624	+	207	Protein_4	DUF905 family protein	-
HYH05_RS25255	3625..4248	+	624	Protein_5	plasmid SOS inhibition protein A	-
HYH05_RS25260	4260..4448	-	189	WP_179226631.1	protein sok	-
-	4260..4484	+	225	NuclAT_0	-	-
-	4260..4484	+	225	NuclAT_0	-	-
-	4260..4484	+	225	NuclAT_0	-	-
-	4260..4484	+	225	NuclAT_0	-	-
-	4417..4482	-	66	-	-	Antitoxin
HYH05_RS25265	4528..4686	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HYH05_RS25270	4924..5301	-	378	Protein_8	hypothetical protein	-
HYH05_RS25275	5601..5897	+	297	WP_001272251.1	hypothetical protein	-
HYH05_RS25280	6008..6829	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HYH05_RS25285	7126..7728	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HYH05_RS25290	8051..8434	+	384	WP_001354030.1	relaxosome protein TraM	-
HYH05_RS25295	8628..9299	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HYH05_RS25300	9436..9663	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / fosA3 / qnrB4 / blaDHA-6 / sul1 / mph(A)	-	1..77194	77194

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T166873 WP_001312861.1 NZ_CP058576:4528-4686 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T166873 NZ_CP074535:c2861449-2860127 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT166873 NZ_CP058576:c4482-4417 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin