Detailed information of TA system TA166869

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 114750..115004
• Replicon: plasmid pEC1-1
• Accession: NZ_CP058575
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HYH05_RS25185
• Protein ID: WP_015387399.1
• Coordinates: 114798..115004 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	114750..114811 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS25150	110403..110615	+	213	WP_005012601.1	hypothetical protein	-
HYH05_RS25155	110916..111005	-	90	Protein_134	IS1 family transposase	-
HYH05_RS25160	111060..111737	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
HYH05_RS25165	111737..112084	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HYH05_RS25170	112104..113675	+	1572	WP_023149734.1	IS66 family transposase	-
HYH05_RS25175	113713..114328	-	616	Protein_138	IS1-like element IS1A family transposase	-
HYH05_RS25180	114429..114611	+	183	WP_000968309.1	hypothetical protein	-
-	114750..114811	-	62	NuclAT_1	-	Antitoxin
-	114750..114811	-	62	NuclAT_1	-	Antitoxin
-	114750..114811	-	62	NuclAT_1	-	Antitoxin
-	114750..114811	-	62	NuclAT_1	-	Antitoxin
HYH05_RS25185	114798..115004	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
HYH05_RS25190	115288..115545	+	258	WP_000083833.1	replication regulatory protein RepA	-
HYH05_RS25195	115781..115855	+	75	WP_032336874.1	RepA leader peptide Tap	-
HYH05_RS25200	115848..116294	+	447	Protein_143	incFII family plasmid replication initiator RepA	-
HYH05_RS25205	116240..116722	-	483	Protein_144	VENN motif pre-toxin domain-containing protein	-
HYH05_RS25210	117614..118834	+	1221	WP_000410951.1	arginine deiminase	-
HYH05_RS25215	118845..119756	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A)	-	1..122379	122379

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T166869 WP_015387399.1 NZ_CP058575:114798-115004 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T166869 NZ_CP074535:350588-350935 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT166869 NZ_CP058575:c114811-114750 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin