Detailed information of TA system TA166838

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 800268..801069
• Replicon: chromosome
• Accession: NZ_CP058574
• Organism: Escherichia coli strain EC1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: HYH05_RS03840
• Protein ID: WP_001094430.1
• Coordinates: 800268..800645 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D3GUT4
• Locus tag: HYH05_RS03845
• Protein ID: WP_001285620.1
• Coordinates: 800692..801069 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH05_RS03815	796399..797382	-	984	WP_001329577.1	KpsF/GutQ family sugar-phosphate isomerase	-
HYH05_RS03820	798193..798363	-	171	Protein_750	IS110 family transposase	-
HYH05_RS03825	798706..799548	-	843	WP_001532553.1	DUF4942 domain-containing protein	-
HYH05_RS03830	799633..799830	-	198	WP_001374283.1	DUF957 domain-containing protein	-
HYH05_RS03835	799842..800271	-	430	Protein_753	hypothetical protein	-
HYH05_RS03840	800268..800645	-	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
HYH05_RS03845	800692..801069	-	378	WP_001285620.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HYH05_RS03850	801232..801453	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HYH05_RS03855	801516..801992	-	477	WP_001186726.1	RadC family protein	-
HYH05_RS03860	802008..802493	-	486	Protein_758	antirestriction protein	-
HYH05_RS03865	802548..803366	-	819	WP_001234620.1	DUF945 domain-containing protein	-
HYH05_RS03870	803466..803699	-	234	WP_001119729.1	DUF905 family protein	-
HYH05_RS03875	803778..804233	-	456	WP_000581504.1	hypothetical protein	-
HYH05_RS03880	804309..804530	-	222	WP_113708371.1	hypothetical protein	-
HYH05_RS03885	804496..804969	-	474	Protein_763	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	kpsF / papI	796399..823474	27075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T166838 WP_001094430.1 NZ_CP058574:c800645-800268 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T166838 NZ_CP074520:c121852-121427 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTGGCTTTGTTGAATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13698.52 Da
• Isoelectric Point: 5.9505

>AT166838 WP_001285620.1 NZ_CP058574:c801069-800692 [Escherichia coli]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT166838 NZ_CP074520:c122079-121849 [Klebsiella pneumoniae]
ATGCGCACAGTATCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7

Antitoxin

• PDB: 2INW
• AlphaFold DB: A0A0E0Y522