Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4069302..4069921 Replicon chromosome
Accession NZ_CP058361
Organism Klebsiella pneumoniae strain KP77

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HYD36_RS20025 Protein ID WP_002892050.1
Coordinates 4069703..4069921 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HYD36_RS20020 Protein ID WP_002892066.1
Coordinates 4069302..4069676 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYD36_RS20010 (4064454) 4064454..4065647 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HYD36_RS20015 (4065670) 4065670..4068816 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HYD36_RS20020 (4069302) 4069302..4069676 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HYD36_RS20025 (4069703) 4069703..4069921 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HYD36_RS20030 (4070080) 4070080..4070646 + 567 WP_002892030.1 maltose O-acetyltransferase -
HYD36_RS20035 (4070618) 4070618..4070758 - 141 WP_004147370.1 hypothetical protein -
HYD36_RS20040 (4070779) 4070779..4071249 + 471 WP_002892026.1 YlaC family protein -
HYD36_RS20045 (4071224) 4071224..4072675 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HYD36_RS20050 (4072776) 4072776..4073474 + 699 WP_002892021.1 GNAT family protein -
HYD36_RS20055 (4073471) 4073471..4073611 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HYD36_RS20060 (4073611) 4073611..4073874 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166731 WP_002892050.1 NZ_CP058361:4069703-4069921 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T166731 NZ_CP074412:591583-591936 [Pseudomonas viridiflava]
ATGCGGGTAATAGCAAAAACGACGGTTGCGAAAGCCATCGAAGCTCATAGTCAGTGGAAGGCGGCACTGAGTTTGTGGCT
GACCACCTTTGACAGGGCGACTCTGCGCTTTGAGTCTTTCGAGCAGCTTCGAGATACCTGGGTCACCACTTCCGGATGGA
ACGTTGACCGAATCCCCTGCTCGAAGCTGAGACAGCCGAGCCTTAAAGGGCCTCTTGATGTGTATGTCTTCGACATCAAG
AAAAATGAGTGCCGGATTATTGCGTGGATTAATACCCGGAATGGCACCATTTATATAAAGGATATTCTGCCCCACGCCGG
TTATGACAAGTGGTGCAAGAGTGACGTTAAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT166731 WP_002892066.1 NZ_CP058361:4069302-4069676 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT166731 NZ_CP074412:591989-592462 [Pseudomonas viridiflava]
ATGAAGCATGATAAGAACGGAGCCACTGGGCTGGATTCCTTCATCGCTAACATTTCACCGATGCTTGATGGCTTAAACGA
TCAGATAGCAACGATGGCCCAGTTATTGGCTGCCTGTCATGATCCCGTCAAGAGTGACACTGACTTGAAGGGGCGGATGG
CGCTGATCAATGAGCTTTACTCTCATGCAGACAGTGAAGATCACGCAGCCGCGCGCTTTGCGGAGCTGGTGGCCGAGCGT
GTGTATGAGTACGAGACCGAAACCGTTTTGATTCCTTACTCCTCTCAGGCGGAAGCGCTGGCTTTCCTGATTTCGGACAG
GGGAGTCAGACAGAAGGATTTGTCAGGTGTTGCCACTCAAAGTGCTGTTTCCGAGATACTGAACAACAAGAGGAAAATGA
CGGTTGCTCAGATAAAAGGCTTTGCAGAATTTTTTAAAGTGCCCGTCGAGTTTTTCATGCATGGTGTCGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References