Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 49115..49950 Replicon chromosome
Accession NZ_CP058355
Organism Escherichia coli strain AMSCJX03

Toxin (Protein)


Gene name yeeV Uniprot ID A0A376WVB1
Locus tag HXS77_RS00250 Protein ID WP_001094448.1
Coordinates 49115..49492 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3T7EC32
Locus tag HXS77_RS00255 Protein ID WP_024175935.1
Coordinates 49582..49950 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS77_RS00225 44480..45403 - 924 WP_000535960.1 carboxylate/amino acid/amine transporter -
HXS77_RS00230 45514..46698 - 1185 WP_001172876.1 sugar efflux transporter -
HXS77_RS00235 47491..48336 - 846 WP_000065751.1 DUF4942 domain-containing protein -
HXS77_RS00240 48421..48618 - 198 WP_000839243.1 DUF957 domain-containing protein -
HXS77_RS00245 48630..49118 - 489 WP_000761714.1 hypothetical protein -
HXS77_RS00250 49115..49492 - 378 WP_001094448.1 TA system toxin CbtA family protein Toxin
HXS77_RS00255 49582..49950 - 369 WP_024175935.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HXS77_RS00260 50024..50245 - 222 WP_000692346.1 DUF987 domain-containing protein -
HXS77_RS00265 50314..50790 - 477 WP_001186709.1 RadC family protein -
HXS77_RS00270 50802..51287 - 486 WP_000214415.1 antirestriction protein -
HXS77_RS00275 51379..52197 - 819 WP_001234749.1 DUF945 domain-containing protein -
HXS77_RS00280 52288..52521 - 234 WP_001117568.1 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14221.22 Da        Isoelectric Point: 8.2919

>T166670 WP_001094448.1 NZ_CP058355:c49492-49115 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Download         Length: 378 bp

>T166670 NZ_CP074366:c4649973-4649662 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13598.40 Da        Isoelectric Point: 6.6255

>AT166670 WP_024175935.1 NZ_CP058355:c49950-49582 [Escherichia coli]
VSDTLSGTTHPDDNDDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT166670 NZ_CP074366:c4649661-4649371 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376WVB1

Antitoxin

Source ID Structure
AlphaFold DB A0A3T7EC32

References