Detailed information of TA system TA166667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4477997..4478727
• Replicon: chromosome
• Accession: NZ_CP058353
• Organism: Serratia marcescens strain ESE2014

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HW877_RS21465
• Protein ID: WP_244757816.1
• Coordinates: 4477997..4478308 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HW877_RS21470
• Protein ID: WP_015379178.1
• Coordinates: 4478305..4478727 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW877_RS21435 (HW877_21420)	4473310..4474035	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
HW877_RS21440 (HW877_21425)	4474088..4475521	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
HW877_RS21445 (HW877_21430)	4475545..4475832	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
HW877_RS21450 (HW877_21435)	4475988..4476449	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
HW877_RS21455 (HW877_21440)	4476579..4477433	+	855	WP_015379177.1	RNase adapter RapZ	-
HW877_RS21460 (HW877_21445)	4477430..4477702	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
HW877_RS21465 (HW877_21450)	4477997..4478308	+	312	WP_244757816.1	type II toxin-antitoxin system HigB family toxin	Toxin
HW877_RS21470 (HW877_21455)	4478305..4478727	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
HW877_RS21475 (HW877_21460)	4478731..4480647	-	1917	WP_048797184.1	PRD domain-containing protein	-
HW877_RS21480 (HW877_21465)	4480699..4481913	-	1215	WP_089185073.1	lactonase family protein	-
HW877_RS21485 (HW877_21470)	4482190..4482930	-	741	WP_047573565.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12293.13 Da
• Isoelectric Point: 10.1871

>T166667 WP_244757816.1 NZ_CP058353:4477997-4478308 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKTLFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHHRRSSS

Nucleotide

• Length: 312 bp

>T166667 NZ_CP074366:3598777-3599319 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT166667 WP_015379178.1 NZ_CP058353:4478305-4478727 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT166667 NZ_CP074366:3598482-3598793 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA