Detailed information of TA system TA166612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2505722..2506360
• Replicon: chromosome
• Accession: NZ_CP058342
• Organism: Escherichia coli strain W60

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: HXP40_RS11845
• Protein ID: WP_001447010.1
• Coordinates: 2506184..2506360 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HXP40_RS11840
• Protein ID: WP_001270286.1
• Coordinates: 2505722..2506138 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXP40_RS11820	2500874..2501815	-	942	WP_001251304.1	ABC transporter permease	-
HXP40_RS11825	2501816..2502829	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
HXP40_RS11830	2502847..2503992	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
HXP40_RS11835	2504237..2505643	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HXP40_RS11840	2505722..2506138	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HXP40_RS11845	2506184..2506360	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HXP40_RS11850	2506582..2506812	+	231	WP_000494244.1	YncJ family protein	-
HXP40_RS11855	2506904..2508865	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HXP40_RS11860	2508938..2509474	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HXP40_RS11865	2509527..2510741	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T166612 WP_001447010.1 NZ_CP058342:c2506360-2506184 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T166612 NZ_CP074359:c1766804-1766430 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT166612 WP_001270286.1 NZ_CP058342:c2506138-2505722 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT166612 NZ_CP074359:c1767261-1766893 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8