Detailed information of TA system TA166326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 701314..701960
• Replicon: chromosome
• Accession: NZ_CP058236
• Organism: Mycobacterium tuberculosis strain 060827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: HWS75_RS03165
• Protein ID: WP_003403137.1
• Coordinates: 701314..701727 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: HWS75_RS03170
• Protein ID: WP_003403139.1
• Coordinates: 701724..701960 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWS75_RS03145	697397..698947	+	1551	WP_003403119.1	MlaD family protein	-
HWS75_RS03150	698999..699391	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
HWS75_RS03155	699388..699645	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
HWS75_RS03160	699828..701063	-	1236	WP_003403128.1	ATP-binding protein	-
HWS75_RS03165	701314..701727	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
HWS75_RS03170	701724..701960	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
HWS75_RS03175	702064..702570	-	507	WP_003900973.1	two component system sensor kinase HK1	-
HWS75_RS03180	702684..703214	-	531	WP_003917320.1	two component system sensor kinase HK2	-
HWS75_RS03185	703198..703893	-	696	WP_003918099.1	two-component system response regulator TcrA	-
HWS75_RS03190	704016..704327	+	312	WP_003403164.1	hypothetical protein	-
HWS75_RS03195	704399..705353	+	955	Protein_634	DUF1259 domain-containing protein	-
HWS75_RS03200	705594..706178	+	585	WP_031667543.1	IS607-like element IS1536 family transposase	-
HWS75_RS03205	706180..706891	+	712	Protein_636	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T166326 WP_003403137.1 NZ_CP058236:c701727-701314 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T166326 NZ_CP074122:33921-34442 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATTCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT166326 WP_003403139.1 NZ_CP058236:c701960-701724 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT166326 NZ_CP074122:33644-33913 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89