Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1905213..1905715 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag HW372_RS08960 Protein ID WP_000767822.1
Coordinates 1905461..1905715 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HW372_RS08955 Protein ID WP_001259255.1
Coordinates 1905213..1905464 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS08930 (1900391) 1900391..1901458 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HW372_RS08935 (1901458) 1901458..1902528 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
HW372_RS08940 (1902525) 1902525..1903829 - 1305 WP_001297912.1 histidinol dehydrogenase -
HW372_RS08945 (1903835) 1903835..1904734 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HW372_RS08950 (1904880) 1904880..1904930 - 51 WP_001364200.1 his operon leader peptide -
HW372_RS08955 (1905213) 1905213..1905464 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HW372_RS08960 (1905461) 1905461..1905715 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HW372_RS08965 (1905798) 1905798..1906622 + 825 WP_000754733.1 SDR family oxidoreductase -
HW372_RS08970 (1906668) 1906668..1907597 + 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
HW372_RS08975 (1907812) 1907812..1907874 + 63 WP_010723108.1 membrane protein YoeI -
HW372_RS08980 (1907864) 1907864..1909222 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T166178 WP_000767822.1 NZ_CP058217:1905461-1905715 [Escherichia coli Nissle 1917]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T166178 NZ_CP074052:3786958-3787176 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT166178 WP_001259255.1 NZ_CP058217:1905213-1905464 [Escherichia coli Nissle 1917]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT166178 NZ_CP074052:3786558-3786932 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References