Detailed information of TA system TA166175

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 842316..843150
• Replicon: chromosome
• Accession: NZ_CP058217
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HW372_RS04090
• Protein ID: WP_000854690.1
• Coordinates: 842316..842693 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HW372_RS04095
• Protein ID: WP_119023792.1
• Coordinates: 842782..843150 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW372_RS04060 (838710)	838710..838880	-	171	Protein_798	IS110 family transposase	-
HW372_RS04065 (839297)	839297..840230	-	934	Protein_799	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HW372_RS04070 (840223)	840223..840618	-	396	WP_000208382.1	DUF6088 family protein	-
HW372_RS04075 (840687)	840687..841532	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
HW372_RS04080 (841617)	841617..841814	-	198	WP_000839293.1	DUF957 domain-containing protein	-
HW372_RS04085 (841831)	841831..842319	-	489	WP_000761699.1	DUF5983 family protein	-
HW372_RS04090 (842316)	842316..842693	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HW372_RS04095 (842782)	842782..843150	-	369	WP_119023792.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
HW372_RS04100 (843200)	843200..843844	-	645	WP_000094916.1	hypothetical protein	-
HW372_RS04105 (843863)	843863..844084	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HW372_RS04110 (844147)	844147..844623	-	477	WP_001186726.1	RadC family protein	-
HW372_RS04115 (844639)	844639..845124	-	486	WP_000849565.1	antirestriction protein	-
HW372_RS04120 (845179)	845179..845997	-	819	WP_001234620.1	DUF932 domain-containing protein	-
HW372_RS04125 (846098)	846098..846331	-	234	WP_000902034.1	DUF905 family protein	-
HW372_RS04130 (846410)	846410..846865	-	456	WP_000581502.1	IrmA family protein	-
HW372_RS04135 (846941)	846941..848068	-	1128	Protein_813	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / sat	822766..890084	67318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T166175 WP_000854690.1 NZ_CP058217:c842693-842316 [Escherichia coli Nissle 1917]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T166175 NZ_CP074052:3128140-3128796 [Escherichia coli]
ATGGCTTATGATTACAGCGCCAGCCTCAACCCGCAAAAAGCTCTGATCTGGCGTATCGTCCATCGGGACAATATCCCGTG
GATTCTGGATAACGGGCTGCACTGCGGCAACAGTCTGGTGCAGGCGGAAAACTGGATCAATATCGGCAACCCGGAGCTGA
TCGGCAAGCGTGCCGGGCATCCGGTACCTGTCGGAACGGGCGGGACACTGCATGACTATGTGCCGTTTTATTTTACCCCC
TTCTCGCCAATGCTGATGAACATCCACAGCGGGCGCGGCGGCATTAAGCAACGCCCCAATGAGGAGATCGTTATTCTGGT
CAGCAACTTGCGAAACGTGGCAGCACACGACGTACCCTTCGTGTTTACCGACAGCCATGCTTACTATAACTGGACCAACT
ACTACACCAGTCTGGATAGTCTGGACCAGATCGACTGGCCCATTTTGCAGGCACGGGACTTTCGCCGCGATCCAGACGAT
CCGGCTAAATTTGAACGCTATCAGGCAGAGGCGCTAATCTGGCAACACTGCCCGATCTCTCTGCTGGATGGGATAATTTG
TTACAGCGAAGAGGTCAGGTTACAATTAGAACAATGGCTGTTTCAGCGTAATCTAACAATGTCCGTTCACACACGGGCTG
GATGGTACTTCTCATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.51 Da
• Isoelectric Point: 4.5851

>AT166175 WP_119023792.1 NZ_CP058217:c843150-842782 [Escherichia coli Nissle 1917]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT166175 NZ_CP074052:3128793-3129860 [Escherichia coli]
ATGATCACATATACACAAGGCAATTTACTGGACGCACCGGTGGAAGCTCTGGTAAATACGGTTAATACCGTCGGTGTCAT
GGGGAAAGGCATCGCGTTGATGTTTAAAGAGCGCTTCCCCGAAAATATGAAAGTCTATGCCCTCGCCTGCAAACAAAAGC
AGGTGATCACCGGCAAAATGTTCATTACCGAAACCGGGGAACTGATGGGACCGCGCTGGATTGTTAATTTTCCGACCAAA
CAGCACTGGCGCGCTGATTCCCGCATGGAGTGGATTGAAGATGGCTTGCAGGACTTACGCCGCTTTTTGATTGAAGAGAA
CGTGCAGTCCATCGCCATTCCACCACTGGGCGCAGGCAACGGTGGTTTGAACTGGCCGGACGTTCGGGCGCAGATTGAAT
CTGCCCTTGGCGACCTTCAGGACGTTGATATCCTGATTTACCAACCGACTGAAAAATACCAGAACGTTGCCAAAAGCACT
GGCGTAAAAAAGCTCACTCCCGCCAGAGCCGCCATCGCTGAACTGGTGCGTCGCTACTGGGTATTGGGGATGGAGTGCAG
CCTACTGGAAATCCAGAAACTGGCCTGGCTGTTACAACGCGCCATTGAGCAGCATCAACAGGACGATATCCTCAAACTGC
GGTTTGAAGCTCACTATTATGGCCCCTATGCGCCCAATCTGAACCATCTGCTTAATGCGCTGGACGGCACCTATCTTAAA
GCCGAGAAACGCATCCCGGACAGCCAACCGCTGGATGTGATCTGGTTTAACGATCAGAAAAAAGAACACGTTAATGCGTA
TCTGAACAACGAAGCCAGAGAGTGGCTACCTGCGCTGGAGCAGGTCAGCCAGTTAATTGACGGCTTTGAATCGCCGTTTG
GTCTGGAGTTGCTGGCAACCGTAGACTGGTTGCTGAGCCGTGGCGAATGTCAACCAACGCTGGATTCCGTTAAGGAAGGG
CTGCACCAGTGGCCTGCGGGCGAACGCTGGGCCAGCCGTAAGTTGAGGCTGTTTGATAATAATAATCTGCAATTTGCCAT
TAACCGCGTAATGGAGTTCCATTGCTGA

Structures

Toxin

• AlphaFold DB: S1PLF5