Detailed information of TA system TA166144

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3989292..3990127
• Replicon: chromosome
• Accession: NZ_CP058209
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HVY12_RS19320
• Protein ID: WP_084560814.1
• Coordinates: 3989750..3990127 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HVY12_RS19315
• Protein ID: WP_139792025.1
• Coordinates: 3989292..3989660 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS19280	3985470..3986006	+	537	WP_001000101.1	DUF4234 domain-containing protein	-
HVY12_RS19285	3986082..3986303	+	222	WP_032216444.1	DUF905 family protein	-
HVY12_RS19290	3986402..3987220	+	819	WP_175097112.1	DUF945 domain-containing protein	-
HVY12_RS19295	3987312..3987797	+	486	WP_084560816.1	antirestriction protein	-
HVY12_RS19300	3987813..3988289	+	477	WP_001186724.1	RadC family protein	-
HVY12_RS19305	3988358..3988579	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HVY12_RS19310	3988598..3989242	+	645	WP_000086753.1	hypothetical protein	-
HVY12_RS19315	3989292..3989660	+	369	WP_139792025.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVY12_RS19320	3989750..3990127	+	378	WP_084560814.1	TA system toxin CbtA family protein	Toxin
HVY12_RS19325	3990124..3990273	+	150	Protein_3765	hypothetical protein	-
HVY12_RS19330	3990349..3990546	+	198	WP_087891818.1	DUF957 domain-containing protein	-
HVY12_RS19335	3990631..3991497	+	867	WP_001290176.1	DUF4942 domain-containing protein	-
HVY12_RS19340	3991569..3991832	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
HVY12_RS19345	3991829..3992155	+	327	WP_000779482.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HVY12_RS19350	3992899..3994434	+	1536	WP_000447015.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC	3955728..3992155	36427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14204.17 Da
• Isoelectric Point: 7.7766

>T166144 WP_084560814.1 NZ_CP058209:3989750-3990127 [Escherichia marmotae]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFIVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T166144 NZ_CP074047:979221-979556 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13759.47 Da
• Isoelectric Point: 5.9618

>AT166144 WP_139792025.1 NZ_CP058209:3989292-3989660 [Escherichia marmotae]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT166144 NZ_CP074047:978973-979221 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA