Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 680049..680728 Replicon chromosome
Accession NZ_CP058157
Organism Enterobacter hormaechei strain RHBSTW-00086

Toxin (Protein)


Gene name ykfI Uniprot ID A0A411GL63
Locus tag HV061_RS03275 Protein ID WP_016243829.1
Coordinates 680387..680728 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A8B2XJW9
Locus tag HV061_RS03270 Protein ID WP_016243830.1
Coordinates 680049..680366 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV061_RS03235 676018..676905 - 888 WP_163415869.1 DMT family transporter -
HV061_RS03240 677018..677350 - 333 WP_111975974.1 gamma-glutamylcyclotransferase -
HV061_RS03245 677469..678368 + 900 WP_111975973.1 transcriptional regulator GcvA -
HV061_RS03250 678641..678832 + 192 Protein_627 DUF905 family protein -
HV061_RS03255 678846..679307 + 462 WP_016239686.1 antirestriction protein -
HV061_RS03260 679316..679798 + 483 WP_032250314.1 DNA repair protein RadC -
HV061_RS03265 679807..680028 + 222 WP_111975972.1 DUF987 domain-containing protein -
HV061_RS03270 680049..680366 + 318 WP_016243830.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV061_RS03275 680387..680728 + 342 WP_016243829.1 TA system toxin CbtA family protein Toxin
HV061_RS03285 681090..681596 + 507 WP_023298636.1 G/U mismatch-specific DNA glycosylase -
HV061_RS03290 681703..683547 - 1845 WP_006812010.1 RNA polymerase sigma factor RpoD -
HV061_RS03295 683701..685446 - 1746 WP_003862574.1 DNA primase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 632487..680728 48241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13020.04 Da        Isoelectric Point: 9.9961

>T165945 WP_016243829.1 NZ_CP058157:680387-680728 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGISLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNSVR

Download         Length: 342 bp

>T165945 NZ_CP074005:1876728-1876979 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12041.72 Da        Isoelectric Point: 7.7518

>AT165945 WP_016243830.1 NZ_CP058157:680049-680366 [Enterobacter hormaechei]
MTHITWGLQRNITPRLGARLVQEGNRMHYLADRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRNAHCVTLFH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT165945 NZ_CP074005:1876976-1877230 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A411GL63

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2XJW9

References