Detailed information of TA system TA165939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4649736..4650352
• Replicon: chromosome
• Accession: NZ_CP058153
• Organism: Enterobacter hormaechei strain RHBSTW-00105

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV071_RS22220
• Protein ID: WP_077266491.1
• Coordinates: 4649736..4650107 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: HV071_RS22225
• Protein ID: WP_015569912.1
• Coordinates: 4650110..4650352 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV071_RS22205	4647236..4648138	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
HV071_RS22210	4648135..4648770	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
HV071_RS22215	4648767..4649696	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
HV071_RS22220	4649736..4650107	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV071_RS22225	4650110..4650352	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV071_RS22230	4650551..4651471	+	921	WP_048983078.1	alpha/beta hydrolase	-
HV071_RS22235	4651480..4652421	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
HV071_RS22240	4652466..4652903	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV071_RS22245	4652900..4653781	-	882	WP_003861949.1	virulence factor BrkB family protein	-
HV071_RS22250	4653775..4654374	-	600	WP_032649898.1	glucose-1-phosphatase	-
HV071_RS22255	4654493..4655293	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T165939 WP_077266491.1 NZ_CP058153:c4650107-4649736 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T165939 NZ_CP074005:c613350-612886 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT165939 WP_015569912.1 NZ_CP058153:c4650352-4650110 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT165939 NZ_CP074005:c613685-613350 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3