Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26338..26981 Replicon plasmid pRHBSTW-00138_2
Accession NZ_CP058132
Organism Klebsiella quasipneumoniae strain RHBSTW-00138

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HV092_RS26195 Protein ID WP_001044770.1
Coordinates 26565..26981 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HV092_RS26190 Protein ID WP_001261282.1
Coordinates 26338..26568 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV092_RS26160 22458..23093 + 636 WP_009309912.1 CPBP family intramembrane metalloprotease -
HV092_RS26165 23201..23545 - 345 WP_009309911.1 hypothetical protein -
HV092_RS26170 23867..24803 + 937 Protein_27 CAAX protease -
HV092_RS26175 24994..25434 - 441 WP_047057603.1 hypothetical protein -
HV092_RS26180 25453..26046 - 594 WP_009309909.1 hypothetical protein -
HV092_RS26185 26145..26381 - 237 Protein_30 hypothetical protein -
HV092_RS26190 26338..26568 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV092_RS26195 26565..26981 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HV092_RS26200 27055..28617 + 1563 WP_009309907.1 AAA family ATPase -
HV092_RS26205 28602..29624 + 1023 WP_000361404.1 helicase UvrD -
HV092_RS26210 30168..31076 + 909 WP_032425603.1 HNH endonuclease -
HV092_RS26215 31262..31612 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..199458 199458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T165883 WP_001044770.1 NZ_CP058132:26565-26981 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T165883 NZ_CP073994:c1853762-1853388 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT165883 WP_001261282.1 NZ_CP058132:26338-26568 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT165883 NZ_CP073994:c1854220-1853852 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References